NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.
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Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG activation. NCP is dual-targeted to plastids and the nucleus. While nuclear NCP mediates the degradation of two repressors of chloroplast biogenesis, PIF1 and PIF3, NCP in plastids promotes the assembly of the PEP complex for PhAPG transcription. NCP and its paralog RCB are non-catalytic thioredoxin-like proteins that diverged in seed plants to adopt nonredundant functions in phytochrome signaling. These results support a model in which phytochromes control PhAPG expression through light-dependent double nuclear and plastidial switches that are linked by evolutionarily conserved and dual-localized regulatory proteins.
Plants, Genetically Modified
DNA-Directed RNA Polymerases
Gene Expression Regulation, Plant
Basic Helix-Loop-Helix Transcription Factors
Published Version (Please cite this version)10.1038/s41467-019-10517-1
Publication InfoYang, Emily J; Yoo, Chan Yul; Liu, Jiangxin; Wang, He; Cao, Jun; Li, Fay-Wei; ... Chen, Meng (2019). NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches. Nature communications, 10(1). pp. 2630. 10.1038/s41467-019-10517-1. Retrieved from https://hdl.handle.net/10161/21729.
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Kathleen M. Pryer
Professor of Biology
Professor of Biology
The diterpenoid phytohormone gibberellin (GA) plays pivotal roles in regulating growth and development throughout the life cycle of higher plants. Mutations affecting GA biosynthesis or GA response were the key to control plant stature in wheat and rice that led to dramatically increased grain yield and contributed greatly to the success of the ‘Green Revolution’ in the 1960s. By multi-faceted approaches using the reference plant Arabidopsis, my lab has made major brea
Professor of Biochemistry
Protein-protein interactions play a pivotal role in the regulation of various cellular processes. The formation of higher order protein complexes is frequently accompanied by extensive structural remodeling of the individual components, varying from domain re-orientation to induced folding of unstructured elements. Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful tool for macromolecular structure determination in solution. It has the unique advantage of being capable of elucidati
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