A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited.
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Premise:Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources-access to a microscope with 1000× magnification is the most limiting factor. Methods and Results:Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos. Conclusions:The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.
Published Version (Please cite this version)10.1002/aps3.11342
Publication InfoWindham, MD; Pryer, KM; Poindexter, DB; Li, F; Rothfels, CJ; & Beck, JB (2020). A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited. Applications in plant sciences, 8(4). pp. e11342. 10.1002/aps3.11342. Retrieved from https://hdl.handle.net/10161/21742.
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