The utility of nuclear gapCp in resolving polyploid fern origins
Abstract
Although polyploidy is rampant in ferns and plays a major role in shaping their diversity,
the evolutionary history of many polyploid species remains poorly understood. Nuclear
DNA sequences can provide valuable information for identifying polyploid origins;
however, remarkably few nuclear markers have been developed specifically for ferns,
and previously published primer sets do not work well in many fern lineages. In this
study, we present new primer sequences for the amplification of a portion of the nuclear
gapCp gene (encoding a glyceraldehyde-3-phosphate dehydrogenase). Through a broad
survey across ferns, we demonstrate that these primers are nearly universal for this
clade. With a case study in cheilanthoids, we show that this rapidly evolving marker
is a powerful tool for discriminating between autopolyploids and allopolyploids. Our
results indicate that gapCp holds considerable potential for addressing species-level
questions across the fern tree of life. © Copyright 2008 by the American Society of
Plant Taxonomists.
Type
Journal articleSubject
Science & TechnologyLife Sciences & Biomedicine
Plant Sciences
Evolutionary Biology
allopolyploidy
autopolyploidy
ferns
gapCp
GAPDH
molecular systematics
nuclear marker
NAD(+)-DEPENDENT GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
PHYLOGENY RECONSTRUCTION
CONCERTED EVOLUTION
GENOME EVOLUTION
DNA-SEQUENCES
RETICULATE EVOLUTION
CHLOROPLAST DNA
RIBOSOMAL DNA
GENE
AMPLIFICATION
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https://hdl.handle.net/10161/21803Published Version (Please cite this version)
10.1600/036364408786500127Publication Info
Schuettpelz, E; Grusz, AL; Windham, MD; & Pryer, KM (2008). The utility of nuclear gapCp in resolving polyploid fern origins. Systematic Botany, 33(4). pp. 621-629. 10.1600/036364408786500127. Retrieved from https://hdl.handle.net/10161/21803.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Kathleen M. Pryer
Professor of Biology

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