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Discrimination of vanadium from zinc using gene profiling in human bronchial epithelial cells.

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Date
2005-12
Authors
Li, Zhuowei
Stonehuerner, Jackie
Devlin, Robert B
Huang, Yuh-Chin T
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Abstract
We hypothesized that gene expression profiling may discriminate vanadium from zinc in human bronchial epithelial cells (HBECs). RNA from HBECs exposed to vehicle, V (50 microM), or Zn (50 microM) for 4 hr (n = 4 paired experiments) was hybridized to Affymetrix Hu133A chips. Using one-class t-test with p < 0.01, we identified 140 and 76 genes with treatment:control ratios > or = 2.0 or < or = 0.5 for V and Zn, respectively. We then categorized these genes into functional pathways and compared the number of genes in each pathway between V and Zn using Fisher's exact test. Three pathways regulating gene transcription, inflammatory response, and cell proliferation distinguished V from Zn. When genes in these three pathways were matched with the 163 genes flagged by the same statistical filtration for V:Zn ratios, 12 genes were identified. The hierarchical clustering analysis showed that these 12 genes discriminated V from Zn and consisted of two clusters. Cluster 1 genes (ZBTB1, PML, ZNF44, SIX1, BCL6, ZNF450) were down-regulated by V and involved in gene transcription, whereas cluster 2 genes (IL8, IL1A, PTGS2, DTR, TNFAIP3, CXCL3) were up-regulated and linked to inflammatory response and cell proliferation. Also, metallothionein 1 genes (MT1F, MT1G, MT1K) were up-regulated by Zn only. Thus, using microarray analysis, we identified a small set of genes that may be used as biomarkers for discriminating V from Zn. The novel genes and pathways identified by the microarray may help us understand the pathogenesis of health effects caused by environmental V and Zn exposure.
Type
Journal article
Subject
Bronchi
Cells, Cultured
Epithelial Cells
Humans
Inflammation
Vanadium
Zinc
Oligonucleotide Array Sequence Analysis
Cluster Analysis
Gene Expression Profiling
Cell Division
Transcription, Genetic
Gene Expression Regulation
Permalink
https://hdl.handle.net/10161/22249
Published Version (Please cite this version)
10.1289/ehp.7947
Publication Info
Li, Zhuowei; Stonehuerner, Jackie; Devlin, Robert B; & Huang, Yuh-Chin T (2005). Discrimination of vanadium from zinc using gene profiling in human bronchial epithelial cells. Environmental health perspectives, 113(12). pp. 1747-1754. 10.1289/ehp.7947. Retrieved from https://hdl.handle.net/10161/22249.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Huang

Yuh-Chin Tony Huang

Professor of Medicine
Closed loop ventilation Environmental medicine Oxidative lung injuryCOPDHyperpolarized 129Xe MRI and regional lung function
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