Discrimination of vanadium from zinc using gene profiling in human bronchial epithelial cells.
Abstract
We hypothesized that gene expression profiling may discriminate vanadium from zinc
in human bronchial epithelial cells (HBECs). RNA from HBECs exposed to vehicle, V
(50 microM), or Zn (50 microM) for 4 hr (n = 4 paired experiments) was hybridized
to Affymetrix Hu133A chips. Using one-class t-test with p < 0.01, we identified 140
and 76 genes with treatment:control ratios > or = 2.0 or < or = 0.5 for V and Zn,
respectively. We then categorized these genes into functional pathways and compared
the number of genes in each pathway between V and Zn using Fisher's exact test. Three
pathways regulating gene transcription, inflammatory response, and cell proliferation
distinguished V from Zn. When genes in these three pathways were matched with the
163 genes flagged by the same statistical filtration for V:Zn ratios, 12 genes were
identified. The hierarchical clustering analysis showed that these 12 genes discriminated
V from Zn and consisted of two clusters. Cluster 1 genes (ZBTB1, PML, ZNF44, SIX1,
BCL6, ZNF450) were down-regulated by V and involved in gene transcription, whereas
cluster 2 genes (IL8, IL1A, PTGS2, DTR, TNFAIP3, CXCL3) were up-regulated and linked
to inflammatory response and cell proliferation. Also, metallothionein 1 genes (MT1F,
MT1G, MT1K) were up-regulated by Zn only. Thus, using microarray analysis, we identified
a small set of genes that may be used as biomarkers for discriminating V from Zn.
The novel genes and pathways identified by the microarray may help us understand the
pathogenesis of health effects caused by environmental V and Zn exposure.
Type
Journal articleSubject
BronchiCells, Cultured
Epithelial Cells
Humans
Inflammation
Vanadium
Zinc
Oligonucleotide Array Sequence Analysis
Cluster Analysis
Gene Expression Profiling
Cell Division
Transcription, Genetic
Gene Expression Regulation
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https://hdl.handle.net/10161/22249Published Version (Please cite this version)
10.1289/ehp.7947Publication Info
Li, Zhuowei; Stonehuerner, Jackie; Devlin, Robert B; & Huang, Yuh-Chin T (2005). Discrimination of vanadium from zinc using gene profiling in human bronchial epithelial
cells. Environmental health perspectives, 113(12). pp. 1747-1754. 10.1289/ehp.7947. Retrieved from https://hdl.handle.net/10161/22249.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Yuh-Chin Tony Huang
Professor of Medicine
Closed loop ventilation Environmental medicine Oxidative lung injuryCOPDHyperpolarized
129Xe MRI and regional lung function

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