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Discrimination of vanadium from zinc using gene profiling in human bronchial epithelial cells.

dc.contributor.author Li, Zhuowei
dc.contributor.author Stonehuerner, Jackie
dc.contributor.author Devlin, Robert B
dc.contributor.author Huang, Yuh-Chin T
dc.date.accessioned 2021-01-26T23:10:17Z
dc.date.available 2021-01-26T23:10:17Z
dc.date.issued 2005-12
dc.identifier.issn 0091-6765
dc.identifier.issn 1552-9924
dc.identifier.uri https://hdl.handle.net/10161/22249
dc.description.abstract We hypothesized that gene expression profiling may discriminate vanadium from zinc in human bronchial epithelial cells (HBECs). RNA from HBECs exposed to vehicle, V (50 microM), or Zn (50 microM) for 4 hr (n = 4 paired experiments) was hybridized to Affymetrix Hu133A chips. Using one-class t-test with p < 0.01, we identified 140 and 76 genes with treatment:control ratios > or = 2.0 or < or = 0.5 for V and Zn, respectively. We then categorized these genes into functional pathways and compared the number of genes in each pathway between V and Zn using Fisher's exact test. Three pathways regulating gene transcription, inflammatory response, and cell proliferation distinguished V from Zn. When genes in these three pathways were matched with the 163 genes flagged by the same statistical filtration for V:Zn ratios, 12 genes were identified. The hierarchical clustering analysis showed that these 12 genes discriminated V from Zn and consisted of two clusters. Cluster 1 genes (ZBTB1, PML, ZNF44, SIX1, BCL6, ZNF450) were down-regulated by V and involved in gene transcription, whereas cluster 2 genes (IL8, IL1A, PTGS2, DTR, TNFAIP3, CXCL3) were up-regulated and linked to inflammatory response and cell proliferation. Also, metallothionein 1 genes (MT1F, MT1G, MT1K) were up-regulated by Zn only. Thus, using microarray analysis, we identified a small set of genes that may be used as biomarkers for discriminating V from Zn. The novel genes and pathways identified by the microarray may help us understand the pathogenesis of health effects caused by environmental V and Zn exposure.
dc.language eng
dc.publisher Environmental Health Perspectives
dc.relation.ispartof Environmental health perspectives
dc.relation.isversionof 10.1289/ehp.7947
dc.subject Bronchi
dc.subject Cells, Cultured
dc.subject Epithelial Cells
dc.subject Humans
dc.subject Inflammation
dc.subject Vanadium
dc.subject Zinc
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject Cluster Analysis
dc.subject Gene Expression Profiling
dc.subject Cell Division
dc.subject Transcription, Genetic
dc.subject Gene Expression Regulation
dc.title Discrimination of vanadium from zinc using gene profiling in human bronchial epithelial cells.
dc.type Journal article
duke.contributor.id Huang, Yuh-Chin T|0100011
dc.date.updated 2021-01-26T23:10:15Z
pubs.begin-page 1747
pubs.end-page 1754
pubs.issue 12
pubs.organisational-group School of Medicine
pubs.organisational-group Medicine, Pulmonary, Allergy, and Critical Care Medicine
pubs.organisational-group Duke
pubs.organisational-group Medicine
pubs.organisational-group Clinical Science Departments
pubs.publication-status Published
pubs.volume 113


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