Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of Caenorhabditis elegans.
Abstract
Standard laboratory culture of Caenorhabditis elegans utilizes solid growth media with a bacterial food source. However, this culture method
limits control of food availability and worm population density, factors that impact
many life-history traits. Here, we describe liquid-culture protocols for precisely
modulating bacterial food availability and population density, facilitating reliable
production of arrested L1 larvae, dauer larvae, dietarily restricted worms, or well-fed
worms. Worms can be grown in small quantities for standard assays or in the millions
for other applications. For complete details on the use and execution of these protocols,
please refer to Hibshman et al. (2016), Webster et al. (2018), and Jordan et al. (2019).
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https://hdl.handle.net/10161/22273Published Version (Please cite this version)
10.1016/j.xpro.2020.100276Publication Info
Hibshman, Jonathan D; Webster, Amy K; & Baugh, L Ryan (2021). Liquid-culture protocols for synchronous starvation, growth, dauer formation, and
dietary restriction of Caenorhabditis elegans. STAR protocols, 2(1). pp. 100276. 10.1016/j.xpro.2020.100276. Retrieved from https://hdl.handle.net/10161/22273.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
L. Ryan Baugh
Professor of Biology
The Baugh Lab is interested in phenotypic plasticity and physiological adaptation
to variable environmental conditions. We are using the roundworm C. elegans to understand
how animals adapt to starvation using primarily genetic and genomic approaches. We
are studying how development is governed by nutrient availability, how animals survive
starvation, and the long-term consequences of starvation including adult disease and
transgenerational epigenetic inheritance.

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