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APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy.

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Date
2019-01
Authors
Bruggeman, Leslie A
Wu, Zhenzhen
Luo, Liping
Madhavan, Sethu
Drawz, Paul E
Thomas, David B
Barisoni, Laura
O'Toole, John F
Sedor, John R
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Abstract
African polymorphisms in the gene for Apolipoprotein L1 (APOL1) confer a survival advantage against lethal trypanosomiasis but also an increased risk for several chronic kidney diseases (CKD) including HIV-associated nephropathy (HIVAN). APOL1 is expressed in renal cells, however, the pathogenic events that lead to renal cell damage and kidney disease are not fully understood. The podocyte function of APOL1-G0 versus APOL1-G2 in the setting of a known disease stressor was assessed using transgenic mouse models. Transgene expression, survival, renal pathology and function, and podocyte density were assessed in an intercross of a mouse model of HIVAN (Tg26) with two mouse models that express either APOL1-G0 or APOL1-G2 in podocytes. Mice that expressed HIV genes developed heavy proteinuria and glomerulosclerosis, and had significant losses in podocyte numbers and reductions in podocyte densities. Mice that co-expressed APOL1-G0 and HIV had preserved podocyte numbers and densities, with fewer morphologic manifestations typical of HIVAN pathology. Podocyte losses and pathology in mice co-expressing APOL1-G2 and HIV were not significantly different from mice expressing only HIV. Podocyte hypertrophy, a known compensatory event to stress, was increased in the mice co-expressing HIV and APOL1-G0, but absent in the mice co-expressing HIV and APOL1-G2. Mortality and renal function tests were not significantly different between groups. APOL1-G0 expressed in podocytes may have a protective function against podocyte loss or injury when exposed to an environmental stressor. This was absent with APOL1-G2 expression, suggesting APOL1-G2 may have lost this protective function.
Type
Journal article
Subject
Kidney Glomerulus
Animals
Mice, Inbred BALB C
Mice, Transgenic
Humans
Mice
AIDS-Associated Nephropathy
Disease Models, Animal
Genetic Predisposition to Disease
Apolipoproteins
Polymorphism, Genetic
Podocytes
Renal Insufficiency, Chronic
Genetic Variation
Transcriptome
Apolipoprotein L1
Permalink
https://hdl.handle.net/10161/22302
Published Version (Please cite this version)
10.1371/journal.pone.0224408
Publication Info
Bruggeman, Leslie A; Wu, Zhenzhen; Luo, Liping; Madhavan, Sethu; Drawz, Paul E; Thomas, David B; ... Sedor, John R (2019). APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy. PloS one, 14(10). pp. e0224408. 10.1371/journal.pone.0224408. Retrieved from https://hdl.handle.net/10161/22302.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Barisoni

Laura Barisoni

Professor of Pathology

David Thomas

Adjunct Professor in the Department of Pathology
Alphabetical list of authors with Scholars@Duke profiles.
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