Stem Cell-Derived Models of Viral Infections in the Gastrointestinal Tract.
Abstract
Studies on the intestinal epithelial response to viral infection have previously been
limited by the absence of in vitro human intestinal models that recapitulate the multicellular
complexity of the gastrointestinal tract. Recent technological advances have led to
the development of "mini-intestine" models, which mimic the diverse cellular nature
and physiological activity of the small intestine. Utilizing adult or embryonic intestinal
tissue, enteroid and organoid systems, respectively, represent an opportunity to effectively
model cellular differentiation, proliferation, and interactions that are specific
to the specialized environment of the intestine. Enteroid and organoid systems represent
a significant advantage over traditional in vitro methods because they model the structure
and function of the small intestine while also maintaining the genetic identity of
the host. These more physiologic models also allow for novel approaches to investigate
the interaction of enteric viruses with the gastrointestinal tract, making them ideal
to study the complexities of host-pathogen interactions in this unique cellular environment.
This review aims to provide a summary on the use of human enteroid and organoid systems
as models to study virus pathogenesis.
Type
Journal articleSubject
Gastrointestinal TractIntestines
Organoids
Stem Cells
Humans
Enterovirus Infections
Models, Biological
Host-Pathogen Interactions
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https://hdl.handle.net/10161/22585Published Version (Please cite this version)
10.3390/v10030124Publication Info
Lanik, Wyatt E; Mara, Madison A; Mihi, Belgacem; Coyne, Carolyn B; & Good, Misty (2018). Stem Cell-Derived Models of Viral Infections in the Gastrointestinal Tract. Viruses, 10(3). pp. 124-124. 10.3390/v10030124. Retrieved from https://hdl.handle.net/10161/22585.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Carolyn Coyne
George Barth Geller Distinguished Professor of Immunology
We study the pathways by which microorganisms cross cellular barriers and the mechanisms
by which these barriers restrict microbial infections. Our studies primarily focus
on the epithelium that lines the gastrointestinal tract and on placental trophoblasts,
the cells that comprise a key cellular barrier of the human placenta. Our work is
highly multidisciplinary and encompasses aspects of cell biology, immunology, and
microbiology. Our long-term goals are to identify pathogen- and host-spe

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