Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds.
Repository Usage Stats
Delivery to the proper tissue compartment is a major obstacle hampering the potential of cellular therapeutics for medical conditions. Delivery of cells within biomaterials may improve localization, but traditional and newer void-forming hydrogels must be made in advance with cells being added into the scaffold during the manufacturing process. Injectable, in situ cross-linking microporous scaffolds are recently developed that demonstrate a remarkable ability to provide a matrix for cellular proliferation and growth in vitro in three dimensions. The ability of these scaffolds to deliver cells in vivo is currently unknown. Herein, it is shown that mesenchymal stem cells (MSCs) can be co-injected locally with microparticle scaffolds assembled in situ immediately following injection. MSC delivery within a microporous scaffold enhances MSC retention subcutaneously when compared to cell delivery alone or delivery within traditional in situ cross-linked nanoporous hydrogels. After two weeks, endothelial cells forming blood vessels are recruited to the scaffold and cells retaining the MSC marker CD29 remain viable within the scaffold. These findings highlight the utility of this approach in achieving localized delivery of stem cells through an injectable porous matrix while limiting obstacles of introducing cells within the scaffold manufacturing process.
Mesenchymal Stem Cells
Mice, Inbred C57BL
Fluorescent Antibody Technique
Published Version (Please cite this version)10.1002/smll.201903147
Publication InfoKoh, Jaekyung; Griffin, Donald R; Archang, Maani M; Feng, An-Chieh; Horn, Thomas; Margolis, Michael; ... Di Carlo, Dino (2019). Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds. Small (Weinheim an der Bergstrasse, Germany), 15(39). pp. e1903147. 10.1002/smll.201903147. Retrieved from https://hdl.handle.net/10161/22635.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
More InfoShow full item record
Professor of Biomedical Engineering
Tatiana Segura is a Professor of Biomedical Engineering, Neurology, and Dermatology at Duke University. She received her B.S. degree in Bioengineering from the University of California, Berkeley (UC Berkeley) and her doctorate in Chemical Engineering from Northwestern University. She began her career in Biomaterials research during her doctoral work working with Prof. Lonnie Shea. She designed hydrogels for local non-viral gene delivery, a topic that she still works on today. She c
Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info