Argon Inhalation for 24 Hours After Onset of Permanent Focal Cerebral Ischemia in Rats Provides Neuroprotection and Improves Neurologic Outcome.
Abstract
<h4>Objectives</h4>We tested the hypothesis that prolonged inhalation of 70% argon
for 24 hours after in vivo permanent or temporary stroke provides neuroprotection
and improves neurologic outcome and overall recovery after 7 days.<h4>Design</h4>Controlled,
randomized, double-blinded laboratory study.<h4>Setting</h4>Animal research laboratories.<h4>Subjects</h4>Adult
Wistar male rats (n = 110).<h4>Interventions</h4>Rats were subjected to permanent
or temporary focal cerebral ischemia via middle cerebral artery occlusion, followed
by inhalation of 70% argon or nitrogen in 30% oxygen for 24 hours. On postoperative
day 7, a 48-point neuroscore and histologic lesion size were assessed.<h4>Measurements
and main results</h4>After argon inhalation for 24 hours immediately following "severe
permanent ischemia" induction, neurologic outcome (neuroscore, p = 0.034), overall
recovery (body weight, p = 0.02), and infarct volume (total infarct volume, p = 0.0001;
cortical infarct volume, p = 0.0003; subcortical infarct volume, p = 0.0001) were
significantly improved. When 24-hour argon treatment was delayed for 2 hours after
permanent stroke induction or until after postischemic reperfusion treatment, neurologic
outcomes remained significantly improved (neuroscore, p = 0.043 and p = 0.014, respectively),
as was overall recovery (body weight, p = 0.015), compared with nitrogen treatment.
However, infarct volume and 7-day mortality were not significantly reduced when argon
treatment was delayed.<h4>Conclusions</h4>Neurologic outcome (neuroscore), overall
recovery (body weight), and infarct volumes were significantly improved after 24-hour
inhalation of 70% argon administered immediately after severe permanent stroke induction.
Neurologic outcome and overall recovery were also significantly improved even when
argon treatment was delayed for 2 hours or until after reperfusion.
Type
Journal articleSubject
AnimalsRats
Rats, Wistar
Brain Ischemia
Disease Models, Animal
Argon
Neuroprotective Agents
Random Allocation
Male
Neuroprotection
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https://hdl.handle.net/10161/23244Published Version (Please cite this version)
10.1097/ccm.0000000000003809Publication Info
Ma, Shuang; Chu, Dongmei; Li, Litao; Creed, Jennifer A; Ryang, Yu-Mi; Sheng, Huaxin;
... Hoffmann, Ulrike (2019). Argon Inhalation for 24 Hours After Onset of Permanent Focal Cerebral Ischemia in
Rats Provides Neuroprotection and Improves Neurologic Outcome. Critical care medicine, 47(8). pp. e693-e699. 10.1097/ccm.0000000000003809. Retrieved from https://hdl.handle.net/10161/23244.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Ulrike Hoffmann
Assistant Professor of Anesthesiology
Huaxin Sheng
Associate Professor in Anesthesiology
We have successfully developed various rodent models of brain and spinal cord injuries
in our lab, such as focal cerebral ischemia, global cerebral ischemia, head trauma,
subarachnoid hemorrhage, intracerebral hemorrhage, spinal cord ischemia and compression
injury. We also established cardiac arrest and hemorrhagic shock models for studying
multiple organ dysfunction. Our current studies focus on two projects. One is to
examine the efficacy of catalytic antioxidant in treating cerebral is
Dennis Alan Turner
Professor of Neurosurgery
Current clinical research interests include clinical trials regarding adaptive or
closed-loop deep brain stimulation with novel devices, cellular, and gene therapy
in Parkinson disease. Additional trials have included gene therapy for Alzheimer's
disease and sensory restoration for development of brain machine interfaces. Clinical
treatments include deep brain stimulation, which is now a common procedure for treating
Parkinson disease and tremor. Translational approaches include testing new devi
David Samuel Warner
Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine
Humans may sustain a variety of forms of acute central nervous system injury including
ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection
Laboratories is dedicated to examining the pathophysiology of acute brain and spinal
cord injury with particular reference to disease states managed in the perioperative
or neurointensive care environments. Rodent recovery models of cerebral ischemia,
traumatic brain injury, cardiopulmonary bypass, subarachnoid he
This author no longer has a Scholars@Duke profile, so the information shown here reflects
their Duke status at the time this item was deposited.
Wei Yang
Associate Professor in Anesthesiology
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