Novel Modification of Potassium Chloride Induced Cardiac Arrest Model for Aged Mice.
Abstract
Experimental cardiac arrest (CA) in aging research is infrequently studied in part
due to the limitation of animal models. We aimed to develop an easily performed mouse
CA model to meet this need. A standard mouse KCl-induced CA model using chest compressions
and intravenous epinephrine for resuscitation was modified by blood withdrawal prior
to CA onset, so as to decrease the requisite KCl dose to induce CA by decreasing the
circulating blood volume. The modification was then compared to the standard model
in young adult mice subjected to 8 min CA. 22-month old mice were then subjected to
8 min CA, resuscitated, and compared to young adult mice. Post-CA functional recovery
was evaluated by measuring spontaneous locomotor activity pre-injury, and on post-CA
days 1, 2, and 3. Neurological score and brain histology were examined on day 3. Brain
elF2α phosphorylation levels were measured at 1 h to verify tissue stress. Compared
to the standard model, the modification decreased cardiopulmonary resuscitation duration
and increased 3-day survival in young mice. For aged mice, survival was 100 % at 24
h and 54% at 72 h. Neurological deficit was present 3 days post-CA, although more
severe versus young mice. Mild neuronal necrosis was present in the cortex and hippocampus.
The modified model markedly induced elF2α phosphorylation in both age groups. This
modified procedure makes the CA model feasible in aged mice and provides a practical
platform for understanding injury mechanisms and developing therapeutics for elderly
patients.
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https://hdl.handle.net/10161/23252Published Version (Please cite this version)
10.14336/ad.2017.0221Publication Info
Liu, Huaqin; Yu, Zhui; Li, Ying; Xu, Bin; Yan, Baihui; Paschen, Wulf; ... Sheng, Huaxin (2018). Novel Modification of Potassium Chloride Induced Cardiac Arrest Model for Aged Mice.
Aging and disease, 9(1). pp. 31-39. 10.14336/ad.2017.0221. Retrieved from https://hdl.handle.net/10161/23252.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Wulf Paschen
Professor in Anesthesiology
My research interests are understanding the mechanisms underlying induction of cell
death induced by a severe form of cellular stress. I am particularly interested in
the role of the endoplasmic reticulum in the pathological process induced by transient
cerebral ischemia and culminating in neuronal cell death. This pathological process
is associated with an irreversible suppression of protein synthese that limits the
ability of cells to withstand ischemia-induced impairment of endoplasmic r
Huaxin Sheng
Associate Professor in Anesthesiology
We have successfully developed various rodent models of brain and spinal cord injuries
in our lab, such as focal cerebral ischemia, global cerebral ischemia, head trauma,
subarachnoid hemorrhage, intracerebral hemorrhage, spinal cord ischemia and compression
injury. We also established cardiac arrest and hemorrhagic shock models for studying
multiple organ dysfunction. Our current studies focus on two projects. One is to
examine the efficacy of catalytic antioxidant in treating cerebral is
David Samuel Warner
Distinguished Distinguished Professor of Anesthesiology, in the School of Medicine
Humans may sustain a variety of forms of acute central nervous system injury including
ischemia, trauma, vasospasm, and perinatal hypoxemia. The Multidisciplinary Neuroprotection
Laboratories is dedicated to examining the pathophysiology of acute brain and spinal
cord injury with particular reference to disease states managed in the perioperative
or neurointensive care environments. Rodent recovery models of cerebral ischemia,
traumatic brain injury, cardiopulmonary bypass, subarachnoid he
This author no longer has a Scholars@Duke profile, so the information shown here reflects
their Duke status at the time this item was deposited.
Wei Yang
Associate Professor in Anesthesiology
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