CometChip enables parallel analysis of multiple DNA repair activities.
Abstract
DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer,
and other diseases. To counteract the deleterious effects of DNA damage, cells have
evolved highly conserved DNA repair pathways. Many commonly used DNA repair assays
are relatively low throughput and are limited to analysis of one protein or one pathway.
Here, we have explored the capacity of the CometChip platform for parallel analysis
of multiple DNA repair activities. Taking advantage of the versatility of the traditional
comet assay and leveraging micropatterning techniques, the CometChip platform offers
increased throughput and sensitivity compared to the traditional comet assay. By exposing
cells to DNA damaging agents that create substrates of Base Excision Repair, Nucleotide
Excision Repair, and Non-Homologous End Joining, we show that the CometChip is an
effective method for assessing repair deficiencies in all three pathways. With these
applications of the CometChip platform, we expand the utility of the comet assay for
precise, high-throughput, parallel analysis of multiple DNA repair activities.
Type
Journal articleSubject
Cell LineCell Line, Tumor
Humans
DNA Damage
DNA
Mutagens
Comet Assay
DNA Repair
High-Throughput Screening Assays
DNA End-Joining Repair
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https://hdl.handle.net/10161/24176Published Version (Please cite this version)
10.1016/j.dnarep.2021.103176Publication Info
Ge, Jing; Ngo, Le P; Kaushal, Simran; Tay, Ian J; Thadhani, Elina; Kay, Jennifer E;
... Engelward, Bevin P (2021). CometChip enables parallel analysis of multiple DNA repair activities. DNA repair, 106. pp. 103176. 10.1016/j.dnarep.2021.103176. Retrieved from https://hdl.handle.net/10161/24176.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Scott Richard Floyd
Gary Hock and Lyn Proctor Associate Professor of Radiation Oncology

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