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How cells determine the number of polarity sites. Chiou, Jian-Geng Moran, Kyle D Lew, Daniel J 2022-03-01T15:13:01Z 2022-03-01T15:13:01Z 2021-04-26
dc.identifier 58768
dc.identifier.issn 2050-084X
dc.identifier.issn 2050-084X
dc.description.abstract The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. A poorly understood but fundamental question concerns the regulatory mechanisms by which different cells generate different numbers of polarity sites. Mass-conserved activator-substrate (MCAS) models that describe polarity circuits develop multiple initial polarity sites, but then those sites engage in competition, leaving a single winner. Theoretical analyses predicted that competition would slow dramatically as GTPase concentrations at different polarity sites increase toward a 'saturation point', allowing polarity sites to coexist. Here, we test this prediction using budding yeast cells, and confirm that increasing the amount of key polarity proteins results in multiple polarity sites and simultaneous budding. Further, we elucidate a novel design principle whereby cells can switch from competition to equalization among polarity sites. These findings provide insight into how cells with diverse morphologies may determine the number of polarity sites.
dc.language eng
dc.publisher eLife Sciences Publications, Ltd
dc.relation.ispartof eLife
dc.relation.isversionof 10.7554/elife.58768
dc.subject Saccharomyces cerevisiae
dc.subject cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
dc.subject Cell Cycle Proteins
dc.subject Cytoskeletal Proteins
dc.subject Saccharomyces cerevisiae Proteins
dc.subject Signal Transduction
dc.subject Cell Division
dc.subject Cell Polarity
dc.subject Cell Shape
dc.subject Gene Expression Regulation, Fungal
dc.subject Models, Biological
dc.subject Time Factors
dc.subject Computer Simulation
dc.subject Numerical Analysis, Computer-Assisted
dc.title How cells determine the number of polarity sites.
dc.type Journal article Lew, Daniel J|0097555 2022-03-01T15:13:01Z
pubs.organisational-group Duke
pubs.organisational-group School of Medicine
pubs.organisational-group Basic Science Departments
pubs.organisational-group Institutes and Centers
pubs.organisational-group Cell Biology
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Pharmacology & Cancer Biology
pubs.organisational-group Duke Cancer Institute
pubs.publication-status Published
pubs.volume 10
duke.contributor.orcid Lew, Daniel J|0000-0001-7482-3585

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