Initial displacement of the intra-articular surface after articular fracture correlates with PTA in C57BL/6 mice but not "superhealer" MRL/MpJ mice.
Abstract
Posttraumatic arthritis (PTA) occurs commonly after articular fracture and may arise,
in part, from joint surface incongruity after injury. MRL/MpJ (MRL) "super-healer"
mice are protected from PTA compared to C57BL/6 (B6) mice following articular fracture.
However, the relationship between the initial displacement of the articular surface,
biologic response, and susceptibility to PTA after fracture remains unclear. The objective
of this study was to assess whether joint incongruity after articular fracture, as
measured by in vivo micro-computed tomography (microCT), could predict pathomechanisms
of PTA in mice. B6 and MRL mice (n = 12/strain) received a closed articular fracture
(fx) of the left tibial plateau. Articular incongruity was quantified as bone surface
deviations (BSD) for each in vivo microCT scan obtained from pre-fx to 8 weeks post-fx,
followed by histologic assessment of arthritis. Serum concentrations of bone formation
(PINP) and bone resorption (CTX-I) biomarkers were quantified longitudinally. Both
strains showed increases in surface incongruity over time, as measured by increases
in BSD. In B6 mice, acute surface incongruity was significantly correlated to the
severity of PTA (R 2 = 0.988; p = .0006), but not in MRL mice (R 2 = 0.224; p = .220). PINP concentrations significantly decreased immediately post-fx
in B6 mice (p = .023) but not in MRL mice, indicating higher bone synthesis in MRL
mice. MRL/MpJ mice demonstrate a unique biologic response to articular fracture such
that the observed articular bone surface displacement does not correlate with the
severity of subsequent PTA. Clinical Relevance: Identifying therapies to enhance acute
biologic repair following articular fracture may mitigate the risk of articular surface
displacement for PTA.
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https://hdl.handle.net/10161/24548Published Version (Please cite this version)
10.1002/jor.24912Publication Info
Vovos, Tyler J; Furman, Bridgette D; Huebner, Janet L; Kimmerling, Kelly A; Utturkar,
Gangadhar M; Green, Cynthia L; ... Olson, Steven A (2021). Initial displacement of the intra-articular surface after articular fracture correlates
with PTA in C57BL/6 mice but not "superhealer" MRL/MpJ mice. Journal of orthopaedic research : official publication of the Orthopaedic Research
Society, 39(9). pp. 1977-1987. 10.1002/jor.24912. Retrieved from https://hdl.handle.net/10161/24548.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Cynthia Lea Green
Associate Professor of Biostatistics & Bioinformatics
Survival Analysis Longitudinal Data Analysis Logistic Regression Missing Data Clinical
Trial Methods Maximum Likelihood Methods
Virginia Byers Kraus
Mary Bernheim Distinguished Professor of Medicine
Virginia Byers Kraus, MD, PhD, is the Mary Bernheim Distinguished Professor of Medicine,
Professor of Orthopaedic Surgery, Professor of Pathology and a faculty member of the
Duke Molecular Physiology Institute in the Duke University School of Medicine. She
is a practicing Rheumatologist with over 30 years’ experience in translational musculoskeletal
research focusing on osteoarthritis, the most common of all arthritides. She trained
at Brown University (ScB 1979), Duke University (MD 19
Steven Arthur Olson
Goldner Jones Distinguished Professor of Orthopaedic Surgery
As an Orthopedic Surgeon my primary focus of research is joint preservation. My primary
clinical interests are Orthopedic Trauma and Hip Reconstruction. In Orthopedic Trauma
my research interests are 1) Basic science investigations of articular fractures with
two current animal models in use. 2) Clinical research includes evaluation of techniques
to reduce and stabilize articular fractures, as well as management of open fractures.
In the area of Hip Reconstruction my ar
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