Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency.
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Oncogenic viruses promote cell proliferation through the dramatic reorganization of host transcriptomes. In addition to regulating mRNA abundance, changes in mRNA isoform usage can have a profound impact on the protein output of the transcriptome. Using Epstein-Barr virus (EBV) transformation of primary B cells, we have studied the ability of an oncogenic virus to alter the mRNA isoform profile of its host. Using the algorithm called SplicerEX with two complementary Affymetrix microarray platforms, we uncovered 433 mRNA isoform changes regulated by EBV during B-cell transformation. These changes were largely orthogonal with the 2,163 mRNA abundance changes observed during transformation, such that less than one-third of mRNAs changing at the level of isoform also changed in overall abundance. While we observed no preference for a mechanistic class of mRNA isoform change, we detected a significant shortening of 3' untranslated regions and exclusion of cassette exons in EBV-transformed cells relative to uninfected B cells. Gene ontology analysis of the mRNA isoform changes revealed significant enrichment in nucleic acid binding proteins. We validated several of these isoform changes and were intrigued by those in two mRNAs encoding the proteins XBP1 and TCF4, which have both been shown to bind and activate the promoter of the major EBV lytic trans-activator BZLF1. Our studies indicate that EBV latent infection promotes the usage of mRNA isoforms of XBP1 and TCF4 that restrict BZLF1 activation. Therefore, characterization of global changes in mRNA isoform usage during EBV infection identifies a new mechanism for the maintenance of latent infection.
Herpesvirus 4, Human
Epstein-Barr Virus Infections
Cell Transformation, Viral
Oligonucleotide Array Sequence Analysis
Gene Expression Profiling
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Viral
Regulatory Sequences, Nucleic Acid
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Promoter Regions, Genetic
Real-Time Polymerase Chain Reaction
Regulatory Factor X Transcription Factors
X-Box Binding Protein 1
Transcription Factor 4
Published Version (Please cite this version)10.1128/jvi.02464-13
Publication InfoHoma, Nicholas J; Salinas, Raul; Forte, Eleonora; Robinson, Timothy J; Garcia-Blanco, Mariano A; & Luftig, Micah A (2013). Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency. Journal of virology, 87(22). pp. 12291-12301. 10.1128/jvi.02464-13. Retrieved from https://hdl.handle.net/10161/24735.
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Mariano Agustin Garcia-Blanco
Adjunct Professor in the Molecular Genetics and Microbiology
Human and viral genes are complex genetic units of information that are tightly regulated. The laboratory studies three aspects of this regulation: the interface between synthesis of mammalian messenger RNAs and the processing events required to mature these transcripts, the alternative processing of these messenger RNAs to produce multiple proteins from one gene, and the regulation of gene expression in human pathogenic flaviviruses. In the great majority of human transcripts
Micah Alan Luftig
Associate Professor of Molecular Genetics and Microbiology
The Luftig laboratory studies viruses that cause cancer with an overarching goal of defining the basic molecular mechanisms underlying pathogenesis and leveraging these findings for diagnostic value and therapeutic intervention. Our work primarily focuses on the common herpesvirus, Epstein-Barr virus (EBV). This virus latently infects virtually all adults worldwide being acquired early in life. In the immune suppressed, EBV promotes lymphomas in the B cells that it naturally infects. However, EB
Research Associate, Senior
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