Post-ischemia common carotid artery occlusion worsens memory loss, but not sensorimotor deficits, in long-term survived stroke mice.
Abstract
Ischemic stroke in rodents is usually induced by intraluminal occlusion of the middle
cerebral artery (MCA) via the external carotid artery (ECA) or the common carotid
artery (CCA). The latter route requires permanent CCA occlusion after ischemia, and
here, we assess its effects on long-term outcomes. Transient occlusion of MCA and
CCA was performed at normal body temperature. After 90 min of ischemia, mice were
randomized to permanent CCA occlusion or no occlusion (control group). Body weight,
and motor and sensory functions, ie, pole test, adhesive tape removal, and elevated
plus maze, were evaluated at 24 h, and at 7 and 28 days after stroke. Infarct volume,
apoptosis, and activation of astrocytes and microglia were assessed at 4 weeks by
an investigator blinded to groups. The Morris water maze test was performed at 3 weeks
in the second experiment. One mouse died at 4 days, and the other mice survived with
persistent neurologic deficits. CCA-occluded mice exhibited delayed turn on the pole
at 24 h and decreased responses to the von Frey filament, and spent more time on the
pole at 7 and 28 days than the control group. Infarction, hemispheric atrophy, glial
activation, and apoptotic neuronal death were present in all mice, and no intra-group
difference was found. However, CCA-occluded mice had a significantly poorer performance
in the Morris water maze compared to the control group, which showed an adverse effect
of post-ischemia CCA occlusion on cognition. Thus, the model selection should be well
considered in preclinical efficacy studies on stroke-induced vascular dementia and
stroke with Alzheimer's disease.
Type
Journal articleSubject
Carotid Artery, CommonAnimals
Mice
Brain Ischemia
Infarction, Middle Cerebral Artery
Amnesia
Ischemia
Disease Models, Animal
Stroke
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https://hdl.handle.net/10161/24850Published Version (Please cite this version)
10.1016/j.brainresbull.2022.03.008Publication Info
Yang, Zhong; Li, Xuan; Cao, Zhipeng; Wang, Peng; Warner, David S; & Sheng, Huaxin (2022). Post-ischemia common carotid artery occlusion worsens memory loss, but not sensorimotor
deficits, in long-term survived stroke mice. Brain research bulletin, 183. pp. 153-161. 10.1016/j.brainresbull.2022.03.008. Retrieved from https://hdl.handle.net/10161/24850.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Huaxin Sheng
Associate Professor in Anesthesiology
We have successfully developed various rodent models of brain and spinal cord injuries
in our lab, such as focal cerebral ischemia, global cerebral ischemia, head trauma,
subarachnoid hemorrhage, intracerebral hemorrhage, spinal cord ischemia and compression
injury. We also established cardiac arrest and hemorrhagic shock models for studying
multiple organ dysfunction. Our current studies focus on two projects. One is to
examine the efficacy of catalytic antioxidant in treating cerebral is

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