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Surgical technique for development of a clinically-representative ventral hernia repair infection rat model.
Abstract
The animal model of infection following ventral hernia repair (VHR) has previously
been utilized in exploring treatments and innovative therapies, such as implantation
of biologic mesh imbedded with various anti-bacterial properties. The rat model has
been utilized most commonly, but prior work has failed to recreate an adequately clinically
representative model of infection following VHR. Additionally, there is lack of standardization
of mesh infection severity across existing literature. Therefore, the aim of this
paper is to describe the creation of a clinically representative VHR infection model
utilizing an index procedure where a hernia defect is created followed by a VHR using
biologic mesh and subsequent infectious agent inoculation. Additionally, we describe
the development of a standardization index to quantify severity of mesh infection:
the Mesh Infection Severity Index (MISI).•Our protocol involves two procedures, an
index procedure where a hernia model is created, and a subsequent procedure where
an infectious inoculant is introduced.•We describe the MISI, a standardization tool
we hope will allow for ease of cross-institutional data assessment.•In summary, our
protocol not only serves as a more clinically representative animal model, but also
includes a novel metric to standardize mesh infection severity.
Type
Journal articlePermalink
https://hdl.handle.net/10161/26288Published Version (Please cite this version)
10.1016/j.mex.2020.100887Publication Info
Anastasio, Albert Thomas; Van Eps, Jeffrey L; & Fernandez-Moure, Joseph S (2020). Surgical technique for development of a clinically-representative ventral hernia repair
infection rat model. MethodsX, 7. pp. 100887. 10.1016/j.mex.2020.100887. Retrieved from https://hdl.handle.net/10161/26288.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Joseph Steven Fernandez-Moure
Assistant Professor of Surgery

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