Bispecific Antibody Therapy for Effective Cardiac Repair through Redirection of Endogenous Stem Cells
Abstract
Bone marrow stem cells (BMSCs) are a promising strategy for cardiac regenerative therapy
for myocardial infarction (MI). However, cell transplantation has to overcome a number
of hurdles, such as cell quality control, clinical practicality, low cell retention/engraftment,
and immune reactions when allogeneic cells are used. Bispecific antibodies (BsAbs)
have been developed as potential agents in cancer immunotherapy but their application
is sparse in cardiovascular diseases. In the present study, BsAbs are designed by
chemical cycloaddition of F(ab′)2 fragments from monoclonal anti-CD34 and anti- cardiac
myosin heavy chain (CMHC) antibodies, which specifically targets circulating CD34-positive
cells and injured cardiomyocytes simultaneously. It is hypothesized that intravenous
administration of stem cell re-directing (SCRD) BsAbs (anti-CD34-F(ab′)2–anti-CMHC-F(ab′)2)
can home endogenous BMSCs to the injured heart for cardiac repair. The in vivo studies
in a mouse model with heart ischemia/reperfusion (I/R) injury demonstrate the safety
and therapeutic potency of SCRD BsAb, which supports cardiac recovery by reducing
scarring, promoting angiomyogenesis, and boosting cardiac function.
Type
Journal articlePermalink
https://hdl.handle.net/10161/26318Published Version (Please cite this version)
10.1002/adtp.201900009Publication Info
Huang, K; Li, Z; Su, T; Shen, D; Hu, S; & Cheng, K (2019). Bispecific Antibody Therapy for Effective Cardiac Repair through Redirection of Endogenous
Stem Cells. Advanced Therapeutics, 2(10). pp. 1900009-1900009. 10.1002/adtp.201900009. Retrieved from https://hdl.handle.net/10161/26318.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
Collections
More Info
Show full item recordScholars@Duke
Teng Su
Assistant Professor in Medicine

Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info