Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.
Abstract
Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared
(NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous
biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano
has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR)
region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano
structure reveals the molecular basis of its red shift. We demonstrate superiority
of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive
tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in
a mouse model of liver inflammation.
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https://hdl.handle.net/10161/26690Published Version (Please cite this version)
10.1038/s41592-022-01683-0Publication Info
(2023). Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared
fluorescent protein. Nature methods, 20(1). pp. 70-74. 10.1038/s41592-022-01683-0. Retrieved from https://hdl.handle.net/10161/26690.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Huaxin Sheng
Associate Professor in Anesthesiology
We have successfully developed various rodent models of brain and spinal cord injuries
in our lab, such as focal cerebral ischemia, global cerebral ischemia, head trauma,
subarachnoid hemorrhage, intracerebral hemorrhage, spinal cord ischemia and compression
injury. We also established cardiac arrest and hemorrhagic shock models for studying
multiple organ dysfunction. Our current studies focus on two projects. One is to
examine the efficacy of catalytic antioxidant in treating cerebral is
Carlos Taboada
Postdoctoral Associate
Junjie Yao
Assistant Professor of Biomedical Engineering
Our mission at PI-Lab is to develop state-of-the-art photoacoustic tomography (PAT)
technologies and translate PAT advances into diagnostic and therapeutic applications,
especially in functional brain imaging and early cancer theranostics. PAT is the most
sensitive modality for imaging rich optical absorption contrast over a wide range
of spatial scales at high speed, and is one of the fastest growing biomedical imaging
technologies. Using numerous endogenous and exogenous contrasts, PAT can
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