Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses.
Abstract
Alterations in the gut microbiota, "dysbiosis," have been reported in autoimmune diseases,
including multiple sclerosis (MS), and their animal models. Although the animal models
were induced by injections of autoantigens with adjuvants, including complete Freund's
adjuvant (CFA) and pertussis toxin (PT), the effects of adjuvant injections on the
microbiota are largely unknown. We aimed to clarify whether adjuvant injections could
affect the microbiota in the ileum and feces. Using 16S rRNA sequencing, we found
decreased alpha diversities of the gut microbiota in mice injected with CFA and PT,
compared with naïve mice. Overall, microbial profiles visualized by principal component
analysis demonstrated dysbiosis in feces, but not in the ileum, of adjuvant-injected
mice, where the genera Lachnospiraceae NK4A136 group and Alistipes contributed to dysbiosis. When we compared the relative abundances of individual
bacteria, we found changes in 16 bacterial genera in feces and seven genera in the
ileum of adjuvant-injected mice, in which increased serum levels of antibody against
mycobacteria (a component of CFA) and total IgG2c were correlated with the genus Facklamia. On the other hand, increased IgG1 and IgA concentrations were correlated with the
genus Atopostipes. Therefore, adjuvant injections alone could alter the overall microbial profiles
(i.e., microbiota) and individual bacterial abundances with altered antibody responses;
dysbiosis in animal models could be partly due to adjuvant injections.
Type
Journal articleSubject
IleumFeces
Animals
Mice
Bacteria
Immunoglobulin G
RNA, Ribosomal, 16S
Adjuvants, Immunologic
Freund's Adjuvant
Anti-Bacterial Agents
Antibody Formation
Dysbiosis
Gastrointestinal Microbiome
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https://hdl.handle.net/10161/27407Published Version (Please cite this version)
10.3390/ijms24032818Publication Info
Khadka, Sundar; Omura, Seiichi; Sato, Fumitaka; & Tsunoda, Ikuo (2023). Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes
in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses. International journal of molecular sciences, 24(3). pp. 2818. 10.3390/ijms24032818. Retrieved from https://hdl.handle.net/10161/27407.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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