Isolation, speciation and antifungal susceptibility testing of Candida isolates from various clinical specimens at a tertiary care hospital, Nepal.
Abstract
<h4>Background</h4>Candida species are responsible for various clinical infections
ranging from mucocutaneous infection to life threatening invasive diseases along with
increased resistance to antifungal drugs has made a serious concern. Resistance to
antifungal agents has increased during the last decade. Thus, identification of Candida
up to species level and its antifungal susceptibility testing has a paramount significance
in the management of Candidal infections. The aim of the study was to speciate Candida
species and to determine antifungal susceptibility pattern of Candida species to antifungal
agents.<h4>Methods</h4>A total of 100 consecutive Candida species were isolated from
1248 clinical specimens over 7 months period. Growths on Sabouraud dextrose agar were
evaluated for colony appearance, macroscopic examination, Gram staining, germ tube
test and urea hydrolysis test. Further, they were processed for Candida speciation
on CHROMagar. Antifungal susceptibility testing was performed as recommended by Clinical
and Laboratory Standards Institute (CLSI) M44-A document.<h4>Results</h4>Out of 100
Candida isolates, Candida albicans (56%) was the most common species. Among the non-albicans
Candida species, Candida tropicalis (20%) was the predominant isolate followed by
Candida glabrata (14%). Regarding antifungal susceptibility pattern, Candida species
were more susceptible to clotrimazole (82%) followed by fluconazole (64%) and miconazole
(44%).<h4>Conclusions</h4>Candida albicans was the predominant species responsible
for various Candidal infections. Among commonly used antifungal drugs clotrimazole,
miconazole and fluconazole were most effective.
Type
Journal articleSubject
HumansCandida albicans
Candida glabrata
Candida tropicalis
Candidiasis
Fluconazole
Clotrimazole
Miconazole
Antifungal Agents
Microbial Sensitivity Tests
Mycological Typing Techniques
Severity of Illness Index
Cross-Sectional Studies
Drug Resistance, Fungal
Nepal
Female
Male
Tertiary Care Centers
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https://hdl.handle.net/10161/27409Published Version (Please cite this version)
10.1186/s13104-017-2547-3Publication Info
Khadka, Sundar; Sherchand, Jeevan Bahadur; Pokhrel, Bharat Mani; Parajuli, Keshab;
Mishra, Shyam Kumar; Sharma, Sangita; ... Rijal, Basista Prasad (2017). Isolation, speciation and antifungal susceptibility testing of Candida isolates from
various clinical specimens at a tertiary care hospital, Nepal. BMC research notes, 10(1). pp. 218. 10.1186/s13104-017-2547-3. Retrieved from https://hdl.handle.net/10161/27409.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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