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Neutralization properties of simian immunodeficiency viruses infecting chimpanzees and gorillas. 

Barbian, Hannah J; Decker, Julie M; Bibollet-Ruche, Frederic; Galimidi, Rachel P; West, Anthony P; Learn, Gerald H; Parrish, Nicholas F; ... (35 authors) (MBio, 2015-04-21)
UNLABELLED: Broadly cross-reactive neutralizing antibodies (bNabs) represent powerful tools to combat human immunodeficiency virus type 1 (HIV-1) infection. Here, we examined whether HIV-1-specific bNabs are capable ...
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Association of HIV-1 Envelope-Specific Breast Milk IgA Responses with Reduced Risk of Postnatal Mother-to-Child Transmission of HIV-1. 

Pollara, Justin; McGuire, Erin; Fouda, Genevieve G; Rountree, Wes; Eudailey, Josh; Overman, R Glenn; Seaton, Kelly E; ... (25 authors) (J Virol, 2015-10)
UNLABELLED: Infants born to HIV-1-infected mothers in resource-limited areas where replacement feeding is unsafe and impractical are repeatedly exposed to HIV-1 throughout breastfeeding. Despite this, the majority of infants ...
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The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved human peripheral blood mononuclear cells. 

Sarzotti-Kelsoe, Marcella; Needham, Leila K; Rountree, Wes; Bainbridge, John; Gray, Clive M; Fiscus, Susan A; Ferrari, Guido; ... (21 authors) (J Immunol Methods, 2014-07)
The Center for HIV/AIDS Vaccine Immunology (CHAVI) consortium was established to determine the host and virus factors associated with HIV transmission, infection and containment of virus replication, with the goal of advancing ...
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Leukopak PBMC sample processing for preparing quality control material to support proficiency testing programs. 

Garcia, Ambrosia; Keinonen, Sarah; Sanchez, Ana M; Ferrari, Guido; Denny, Thomas N; Moody, M Anthony (J Immunol Methods, 2014-07)
External proficiency testing programs designed to evaluate the performance of end-point laboratories involved in vaccine and therapeutic clinical trials form an important part of clinical trial quality assurance. Good clinical ...
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Introduction to a Special Issue of the Journal of Immunological Methods: Building global resource programs to support HIV/AIDS clinical trial studies. 

Sanchez, Ana M; Denny, Thomas N; O'Gorman, Maurice (J Immunol Methods, 2014-07)
This Special Issue of the Journal of Immunological Methods includes 16 manuscripts describing quality assurance activities related to virologic and immunologic monitoring of six global laboratory resource programs that support ...
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Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays 

Staats, Janet S; Enzor, Jennifer H; Sanchez, Ana M; Rountree, Wes; Chan, Cliburn; Jaimes, Maria; Chan, Ray Chun-Fai; ... (10 authors) (Journal of Immunological Methods, 2014-01-01)
The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular ...
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The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk. 

Mahlokozera, Tatenda; Kang, Helen H; Goonetilleke, Nilu; Stacey, Andrea R; Lovingood, Rachel V; Denny, Thomas N; Kalilani, Linda; ... (13 authors) (PLoS One, 2011)
BACKGROUND: The risk of postnatal HIV transmission is associated with the magnitude of the milk virus load. While HIV-specific cellular immune responses control systemic virus load and are detectable in milk, the contribution ...
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Elucidation of hepatitis C virus transmission and early diversification by single genome sequencing. 

Li, Hui; Stoddard, Mark B; Wang, Shuyi; Blair, Lily M; Giorgi, Elena E; Parrish, Erica H; Learn, Gerald H; ... (18 authors) (PLoS Pathog, 2012)
A precise molecular identification of transmitted hepatitis C virus (HCV) genomes could illuminate key aspects of transmission biology, immunopathogenesis and natural history. We used single genome sequencing of 2,922 half ...
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Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated. 

Liao, Hua-Xin; Chen, Xi; Munshaw, Supriya; Zhang, Ruijun; Marshall, Dawn J; Vandergrift, Nathan; Whitesides, John F; ... (32 authors) (J Exp Med, 2011-10-24)
The initial antibody response to HIV-1 is targeted to envelope (Env) gp41, and is nonneutralizing and ineffective in controlling viremia. To understand the origins and characteristics of gp41-binding antibodies produced ...
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Initial HIV-1 antigen-specific CD8+ T cells in acute HIV-1 infection inhibit transmitted/founder virus replication. 

Freel, Stephanie A; Picking, Ralph A; Ferrari, Guido; Ding, Haitao; Ochsenbauer, Christina; Kappes, John C; Kirchherr, Jennifer L; ... (16 authors) (J Virol, 2012-06)
CD8-mediated virus inhibition can be detected in HIV-1-positive subjects who naturally control virus replication. Characterizing the inhibitory function of CD8(+) T cells during acute HIV-1 infection (AHI) can elucidate ...
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Denny, Thomas N (49)
Tomaras, Georgia D (15)Haynes, Barton F (13)Sanchez, Ana M (13)Ferrari, Guido (11)Rountree, Wes (11)Montefiori, David C (9)Liao, Hua-Xin (8)Moody, M Anthony (8)Permar, Sallie R (8)... View MoreDate Issued2014 (14)2011 (7)2015 (5)2013 (4)2016 (4)2017 (4)2010 (3)2012 (3)2018 (3)2019 (2)TypeJournal article (48)Other article (1)SubjectHumans (35)HIV Infections (23)HIV-1 (20)Female (15)HIV Antibodies (10)Biomarkers (9)Reproducibility of Results (9)Guideline Adherence (8)Observer Variation (8)Practice Guidelines as Topic (8)... View MoreAffiliation of Duke Author(s)
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