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Distinctive variation in the U3R region of the 5' Long Terminal Repeat from diverse HIV-1 strains.
(PloS one, 2018-01)
Functional mapping of the 5'LTR has shown that the U3 and the R regions (U3R) contain
a cluster of regulatory elements involved in the control of HIV-1 transcription and
expression. As the HIV-1 genome is characterized by ...
Development of a contemporary globally diverse HIV viral panel by the EQAPOL program.
(J Immunol Methods, 2014-07)
The significant diversity among HIV-1 variants poses serious challenges for vaccine
development and for developing sensitive assays for screening, surveillance, diagnosis,
and clinical management. Recognizing a need to develop ...
Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays.
(J Immunol Methods, 2014-07)
The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry
Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other
interested research laboratories in performing Intracellular ...
Association of HIV-1 Envelope-Specific Breast Milk IgA Responses with Reduced Risk of Postnatal Mother-to-Child Transmission of HIV-1.
(J Virol, 2015-10)
UNLABELLED: Infants born to HIV-1-infected mothers in resource-limited areas where
replacement feeding is unsafe and impractical are repeatedly exposed to HIV-1 throughout
breastfeeding. Despite this, the majority of infants ...
HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies.
(J Virol, 2011-09)
Despite months of mucosal virus exposure, the majority of breastfed infants born to
HIV-infected mothers do not become infected, raising the possibility that immune factors
in milk inhibit mucosal transmission of HIV. HIV ...
A model for harmonizing flow cytometry in clinical trials.
(Nat Immunol, 2010-11)
Complexities in sample handling, instrument setup and data analysis are barriers to
the effective use of flow cytometry to monitor immunological parameters in clinical
trials. The novel use of a central laboratory may help ...
Maternal HIV-1 envelope-specific antibody responses and reduced risk of perinatal transmission.
(J Clin Invest, 2015-07-01)
Despite the wide availability of antiretroviral drugs, more than 250,000 infants are
vertically infected with HIV-1 annually, emphasizing the need for additional interventions
to eliminate pediatric HIV-1 infections. Here, ...
Setting objective thresholds for rare event detection in flow cytometry.
(J Immunol Methods, 2014-07)
The accurate identification of rare antigen-specific cytokine positive cells from
peripheral blood mononuclear cells (PBMC) after antigenic stimulation in an intracellular
staining (ICS) flow cytometry assay is challenging, ...
Leukopak PBMC sample processing for preparing quality control material to support proficiency testing programs.
(J Immunol Methods, 2014-07)
External proficiency testing programs designed to evaluate the performance of end-point
laboratories involved in vaccine and therapeutic clinical trials form an important
part of clinical trial quality assurance. Good clinical ...
Comparison of interlaboratory variation in absolute T-cell counts by single-platform and optimized dual-platform methods.
(Cytometry B Clin Cytom, 2010-05)
BACKGROUND: Previous studies have reported that the adoption of a single-platform
flow cytometry cell counting method resulted in lower interlaboratory variation in
absolute T cell counts as compared to predicate dual-platform ...