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The effects of osmotic stress on the structure and function of the cell nucleus.

dc.contributor.advisor Guilak, Farshid
dc.contributor.advisor Kraus, Virginia
dc.contributor.advisor Setton, Lori A.
dc.contributor.advisor Wax, Adam P.
dc.contributor.advisor Zauscher, Stefan
dc.contributor.author Finan, John Desmond
dc.coverage.spatial United States
dc.date.accessioned 2011-01-05T14:40:04Z
dc.date.issued 2010
dc.identifier.uri https://hdl.handle.net/10161/2974
dc.description.abstract Chondrocytes maintain cartilage by transducing joint load into appropriate biosynthetic activity, a process commonly known as mechanotransduction. Malfunctioning mechanotransduction leads to cartilage degradation and osteoarthritis. The mechanism of mechanotransduction is only partially understood but osmotic stresses are thought to play an important role. This study shows that the chondrocyte nucleus shrinks and wrinkles under hyper-osmotic stress. It shrinks because the chromatin inside the nucleus contracts as the macromolecules in the cell become more crowded. It wrinkles because the nuclear lamina buckles as the nucleus contracts. These morphological changes accelerate transport across the nuclear envelope. Many cells have organized actin caps around their nuclei that constrain the nucleus from contracting under hyper-osmotic stress. Agents exist that can reverse this loss of osmotic sensitivity in vitro without damaging the cell.
dc.language eng
dc.subject Animals
dc.subject Cell Nucleus
dc.subject Humans
dc.subject Osmolar Concentration
dc.subject Osmosis
dc.subject Osmotic Pressure
dc.subject Signal Transduction
dc.title The effects of osmotic stress on the structure and function of the cell nucleus.
dc.type Dissertation
dc.department Biomedical Engineering
duke.embargo.months 24
pubs.organisational-group Duke
pubs.organisational-group Duke
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Duke
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Biomedical Engineering
pubs.publication-status Published


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