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Characterization of Metal Binding Peptides Derived from Copper Trafficking Proteins

dc.contributor.advisor Franz, Katherine J Rubino, Jeffrey Tyler 2011-01-05T14:40:13Z 2012-09-01T04:30:08Z 2010
dc.description.abstract <p>Copper was first released into the environment as the result of the mass generation of oxygen from photosynthetic bacteria roughly 2.7 billion years ago. While it proved to be poisonous to early life on Earth, those that met the evolutionary challenge utilized the metal as a cofactor in enzymes to perform biochemically significant functions, while controlling intracellular levels of copper with a sophisticated network of trafficking proteins. Proteins and enzymes that utilize copper as a cofactor have evolved significantly different coordination environments than copper trafficking proteins, as a result of the different functions they perform. Of particular interest was characterizing the unique Cu(I) binding events observed in some of these proteins, the extracellular N-terminal regions of eukaryotic high affinity copper transport proteins (Ctr), and the bacterial periplamsic CusF protein of the CusFBCA Cu(I)/Ag(I) efflux pathway. </p><p>Model peptides corresponding to the methionine rich binding motifs (Mets motifs) were characterized in terms of Cu(I) binding affinity, stoichiometry, and metal specificity, via an ascorbic acid oxidation assay and electrospray ionization mass spectrometry. Metal induced structural features and coordination environments were elucidated with NMR, CD and X-ray spectroscopy. A series of peptides was also examined to infer the relative Cu(I) binding affinities, and susceptibility to oxidation, of methionine, histidine, cysteine residues found in copper binding motifs. The resistance of Cu+ specific peptides to metal catalyzed oxidation is also described. Attempts were also made to model the Cu(I)/Ag(I) tryptophan cation-&#960; interaction observed in CusF.</p>
dc.subject Chemistry, Inorganic
dc.subject Copper
dc.subject Copper transport
dc.subject Ctr
dc.subject Methionine
dc.subject Peptides
dc.subject Silver
dc.title Characterization of Metal Binding Peptides Derived from Copper Trafficking Proteins
dc.type Dissertation
dc.department Chemistry
duke.embargo.months 24

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