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Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells
Abstract
Interleukin-17 (IL-17) is a cytokine recently shown to be elevated, along with interferon-γ
(IFNγ) and tumor necrosis factor (TNFα), in degenerated and herniated intervertebral
disc (IVD) tissues, suggesting a role for these cytokines in intervertebral disc disease.
The objective of our study was to investigate the involvement of IL-17 and costimulants
IFNγ and TNFα in intervertebral disc pathology. Cells were isolated from anulus fibrosus
and nucleus pulposus tissues of patients undergoing surgery for intervertebral disc
degeneration or scoliosis. The production of inflammatory mediators, nitric oxide
(NOx), prostaglandin E2 (PGE2) and interleukin-6 (IL-6), as well as intercellular
adhesion molecule (ICAM-1) expression, were quantified for cultured cells following
exposure to IL-17, IFNγ and TNFα. Intervertebral disc cells exposed to IL-17, IFNγ
or TNFα showed a remarkable increase in inflammatory mediator release and ICAM-1 expression
(GLM and ANOVA, p<0.05). Addition of IFNγ or TNFα to IL-17 demonstrated a synergistic
increase in inflammatory mediator release, and a marked increase in ICAM-1 expression.
These findings suggest that IVD cells not only respond with a catabolic phenotype
to IL-17 and costimulants IFNγ and TNFα, but also express surface ligands with consequent
potential to recruit additional lymphocytes and immune cells to the IVD microenvironment.
IL-17 may be an important regulator of inflammation in the IVD pathologies.
Type
Journal articlePermalink
https://hdl.handle.net/10161/3167Published Version (Please cite this version)
10.1002/jor.2120Publication Info
Allen, Kyle D; Chen, Jun; Fitch, Robert; Gabr, Mostafa A; Helbling, Antonia R; Jing,
Liufang; ... Sinclair, S Michael (2011). Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release
and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral
disc cells. 10.1002/jor.2120. Retrieved from https://hdl.handle.net/10161/3167.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
William James Richardson
Professor of Orthopaedic Surgery
1. Current research includes investigation of biomechanical aspects of cervical injury
with head impact. This involves cadaveric work with high-speed photography and load
cells to ascertain the mechanism for spinal fractures. 2. An animal model is being
used to evaluate the biomechanics of cervical laminectomy versus laminoplasty compared
to the normal spine. A portion of the animals are developing myelopathy secondary
to instability after the surgical procedure and this is being ev

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