Chondrogenesis of adult stem cells from adipose tissue and bone marrow: induction by growth factors and cartilage-derived matrix.
Abstract
OBJECTIVES: Adipose-derived stem cells (ASCs) and bone marrow-derived mesenchymal
stem cells (MSCs) are multipotent adult stem cells with potential for use in cartilage
tissue engineering. We hypothesized that these cells show distinct responses to different
chondrogenic culture conditions and extracellular matrices, illustrating important
differences between cell types. METHODS: Human ASCs and MSCs were chondrogenically
differentiated in alginate beads or a novel scaffold of reconstituted native cartilage-derived
matrix with a range of growth factors, including dexamethasone, transforming growth
factor beta3, and bone morphogenetic protein 6. Constructs were analyzed for gene
expression and matrix synthesis. RESULTS: Chondrogenic growth factors induced a chondrocytic
phenotype in both ASCs and MSCs in alginate beads or cartilage-derived matrix. MSCs
demonstrated enhanced type II collagen gene expression and matrix synthesis as well
as a greater propensity for the hypertrophic chondrocyte phenotype. ASCs had higher
upregulation of aggrecan gene expression in response to bone morphogenetic protein
6 (857-fold), while MSCs responded more favorably to transforming growth factor beta3
(573-fold increase). CONCLUSIONS: ASCs and MSCs are distinct cell types as illustrated
by their unique responses to growth factor-based chondrogenic induction. This chondrogenic
induction is affected by the composition of the scaffold and the presence of serum.
Type
Journal articleSubject
Adipose TissueAdult
Adult Stem Cells
Alginates
Bone Marrow Cells
Bone Morphogenetic Protein 6
Cartilage
Chondrogenesis
DNA
Extracellular Matrix
Extracellular Matrix Proteins
Female
Gene Expression Regulation
Glucuronic Acid
Glycosaminoglycans
Hexuronic Acids
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins
Reverse Transcriptase Polymerase Chain Reaction
Tissue Scaffolds
Transforming Growth Factor beta
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https://hdl.handle.net/10161/3329Published Version (Please cite this version)
10.1089/ten.TEA.2009.0398Publication Info
Diekman, Brian O; Rowland, Christopher R; Lennon, Donald P; Caplan, Arnold I; & Guilak,
Farshid (2010). Chondrogenesis of adult stem cells from adipose tissue and bone marrow: induction
by growth factors and cartilage-derived matrix. Tissue Eng Part A, 16(2). pp. 523-533. 10.1089/ten.TEA.2009.0398. Retrieved from https://hdl.handle.net/10161/3329.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Farshid Guilak
Lazlo Ormandy Professor of Orthopaedic Surgery
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