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Chondrogenesis of adult stem cells from adipose tissue and bone marrow: induction by growth factors and cartilage-derived matrix.

dc.contributor.author Caplan, AI
dc.contributor.author Diekman, BO
dc.contributor.author Guilak, Farshid
dc.contributor.author Lennon, DP
dc.contributor.author Rowland, CR
dc.coverage.spatial United States
dc.date.accessioned 2011-04-15T16:46:34Z
dc.date.issued 2010-02
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/19715387
dc.identifier.uri http://hdl.handle.net/10161/3329
dc.description.abstract OBJECTIVES: Adipose-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells with potential for use in cartilage tissue engineering. We hypothesized that these cells show distinct responses to different chondrogenic culture conditions and extracellular matrices, illustrating important differences between cell types. METHODS: Human ASCs and MSCs were chondrogenically differentiated in alginate beads or a novel scaffold of reconstituted native cartilage-derived matrix with a range of growth factors, including dexamethasone, transforming growth factor beta3, and bone morphogenetic protein 6. Constructs were analyzed for gene expression and matrix synthesis. RESULTS: Chondrogenic growth factors induced a chondrocytic phenotype in both ASCs and MSCs in alginate beads or cartilage-derived matrix. MSCs demonstrated enhanced type II collagen gene expression and matrix synthesis as well as a greater propensity for the hypertrophic chondrocyte phenotype. ASCs had higher upregulation of aggrecan gene expression in response to bone morphogenetic protein 6 (857-fold), while MSCs responded more favorably to transforming growth factor beta3 (573-fold increase). CONCLUSIONS: ASCs and MSCs are distinct cell types as illustrated by their unique responses to growth factor-based chondrogenic induction. This chondrogenic induction is affected by the composition of the scaffold and the presence of serum.
dc.language eng
dc.language.iso en_US
dc.relation.ispartof Tissue Eng Part A
dc.relation.isversionof 10.1089/ten.TEA.2009.0398
dc.subject Adipose Tissue
dc.subject Adult
dc.subject Adult Stem Cells
dc.subject Alginates
dc.subject Bone Marrow Cells
dc.subject Bone Morphogenetic Protein 6
dc.subject Cartilage
dc.subject Chondrogenesis
dc.subject DNA
dc.subject Extracellular Matrix
dc.subject Extracellular Matrix Proteins
dc.subject Female
dc.subject Gene Expression Regulation
dc.subject Glucuronic Acid
dc.subject Glycosaminoglycans
dc.subject Hexuronic Acids
dc.subject Humans
dc.subject Immunohistochemistry
dc.subject Intercellular Signaling Peptides and Proteins
dc.subject Reverse Transcriptase Polymerase Chain Reaction
dc.subject Tissue Scaffolds
dc.subject Transforming Growth Factor beta
dc.title Chondrogenesis of adult stem cells from adipose tissue and bone marrow: induction by growth factors and cartilage-derived matrix.
dc.type Journal article
dc.description.version Version of Record
duke.date.pubdate 2010-2-0
duke.description.issue 2
duke.description.volume 16
dc.relation.journal Tissue Engineering Part a
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/19715387
pubs.begin-page 523
pubs.end-page 533
pubs.issue 2
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Duke
pubs.organisational-group Pratt School of Engineering
pubs.publication-status Published
pubs.volume 16
dc.identifier.eissn 1937-335X


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