Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.
Abstract
Articular cartilage possesses complex mechanical properties that provide healthy joints
the ability to bear repeated loads and maintain smooth articulating surfaces over
an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold
designed to mimic the anisotropic, nonlinear, and viscoelastic biomechanical characteristics
of native cartilage as the basis for developing functional tissue-engineered constructs.
Three-dimensionally woven poly(epsilon-caprolactone) (PCL) scaffolds were encapsulated
with a fibrin hydrogel, seeded with human adipose-derived stem cells, and cultured
for 28 days in chondrogenic culture conditions. Biomechanical testing showed that
PCL-based constructs exhibited baseline compressive and shear properties similar to
those of native cartilage and maintained these properties throughout the culture period,
while supporting the synthesis of a collagen-rich extracellular matrix. Further, constructs
displayed an equilibrium coefficient of friction similar to that of native articular
cartilage (mu(eq) approximately 0.1-0.3) over the prescribed culture period. Our findings
show that three-dimensionally woven PCL-fibrin composite scaffolds can be produced
with cartilage-like mechanical properties, and that these engineered properties can
be maintained in culture while seeded stem cells regenerate a new, functional tissue
construct.
Type
Journal articleSubject
Adult Stem CellsBiocompatible Materials
Biomechanical Phenomena
Cartilage, Articular
Chondrocytes
Chondrogenesis
Collagen
Compressive Strength
Extracellular Matrix
Fibrin
Friction
Humans
Hydrogels
Materials Testing
Microscopy, Electron, Scanning
Polyesters
Regeneration
Shear Strength
Tensile Strength
Tissue Engineering
Tissue Scaffolds
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https://hdl.handle.net/10161/3355Published Version (Please cite this version)
10.1089/ten.TEA.2009.0480Publication Info
Moutos, Franklin T; & Guilak, Farshid (2010). Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone)
scaffolds for cartilage tissue engineering. Tissue Eng Part A, 16(4). pp. 1291-1301. 10.1089/ten.TEA.2009.0480. Retrieved from https://hdl.handle.net/10161/3355.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Farshid Guilak
Lazlo Ormandy Professor of Orthopaedic Surgery
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