Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells.
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Human adipose stem cells (hASCs) can differentiate into a variety of phenotypes. Native extracellular matrix (e.g., demineralized bone matrix or small intestinal submucosa) can influence the growth and differentiation of stem cells. The hypothesis of this study was that a novel ligament-derived matrix (LDM) would enhance expression of a ligamentous phenotype in hASCs compared to collagen gel alone. LDM prepared using phosphate-buffered saline or 0.1% peracetic acid was mixed with collagen gel (COL) and was evaluated for its ability to induce proliferation, differentiation, and extracellular matrix synthesis in hASCs over 28 days in culture at different seeding densities (0, 0.25 x 10(6), 1 x 10(6), or 2 x 10(6) hASC/mL). Biochemical and gene expression data were analyzed using analysis of variance. Fisher's least significant difference test was used to determine differences between treatments following analysis of variance. hASCs in either LDM or COL demonstrated changes in gene expression consistent with ligament development. hASCs cultured with LDM demonstrated more dsDNA content, sulfated-glycosaminoglycan accumulation, and type I and III collagen synthesis, and released more sulfated-glycosaminoglycan and collagen into the medium compared to hASCs in COL (p <or= 0.05). Increased seeding density increased DNA content incrementally over 28 days in culture for LDM but not COL constructs (p <or= 0.05). These findings suggest that LDM can stimulate a ligament phenotype by hASCs, and may provide a novel scaffold material for ligament engineering applications.
Gene Expression Regulation
Published Version (Please cite this version)10.1089/ten.tea.2009.0720
Publication InfoLittle, Dianne; Guilak, Farshid; & Ruch, David S (2010). Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells. Tissue Eng Part A, 16(7). pp. 2307-2319. 10.1089/ten.tea.2009.0720. Retrieved from https://hdl.handle.net/10161/3364.
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Lazlo Ormandy Professor of Orthopaedic Surgery
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.
Assistant Professor in Orthopaedic Surgery
BVSc (DVM Equivalent) University of Liverpool 1998MSpVM North Carolina State University 2003PhD (Physiology and Biotechnology) North Carolina State University 2006Diplomate, American College of Veterinary Surgeons 2004My research focus is rotator cuff tendon tissue engineering and tendon-bone interfacial regeneration using a novel electrospinning technology. I have also developed an interest in integrated 'omics to assess continued risk for tendon degeneration
Professor of Orthopaedic Surgery
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