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Effect of microRNA modulation on bioartificial muscle function.

dc.contributor.author Rhim, Caroline
dc.contributor.author Cheng, Cindy S
dc.contributor.author Kraus, William E
dc.contributor.author Truskey, George A
dc.coverage.spatial United States
dc.date.accessioned 2011-04-15T16:46:36Z
dc.date.issued 2010-12
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20670163
dc.identifier.uri https://hdl.handle.net/10161/3365
dc.description.abstract Cellular therapies have recently employed the use of small RNA molecules, particularly microRNAs (miRNAs), to regulate various cellular processes that may be altered in disease states. In this study, we examined the effect of transient muscle-specific miRNA inhibition on the function of three-dimensional skeletal muscle cultures, or bioartificial muscles (BAMs). Skeletal myoblast differentiation in vitro is enhanced by inhibiting a proliferation-promoting miRNA (miR-133) expressed in muscle tissues. As assessed by functional force measurements in response to electrical stimulation at frequencies ranging from 0 to 20 Hz, peak forces exhibited by BAMs with miR-133 inhibition (anti-miR-133) were on average 20% higher than the corresponding negative control, although dynamic responses to electrical stimulation in miRNA-transfected BAMs and negative controls were similar to nontransfected controls. Immunostaining for alpha-actinin and myosin also showed more distinct striations and myofiber organization in anti-miR-133 BAMs, and fiber diameters were significantly larger in these BAMs over both the nontransfected and negative controls. Compared to the negative control, anti-miR-133 BAMs exhibited more intense nuclear staining for Mef2, a key myogenic differentiation marker. To our knowledge, this study is the first to demonstrate that miRNA mediation has functional effects on tissue-engineered constructs.
dc.language eng
dc.language.iso en_US
dc.publisher Mary Ann Liebert Inc
dc.relation.ispartof Tissue Eng Part A
dc.relation.isversionof 10.1089/ten.TEA.2009.0601
dc.subject Actinin
dc.subject Animals
dc.subject Cell Differentiation
dc.subject Cell Line
dc.subject Cell Proliferation
dc.subject Mice
dc.subject MicroRNAs
dc.subject Muscle, Skeletal
dc.subject Myoblasts, Skeletal
dc.subject Myosins
dc.subject Tissue Engineering
dc.title Effect of microRNA modulation on bioartificial muscle function.
dc.type Journal article
duke.contributor.id Kraus, William E|0078469
duke.contributor.id Truskey, George A|0099592
dc.description.version Version of Record
duke.date.pubdate 2010-12-0
duke.description.issue 12
duke.description.volume 16
dc.relation.journal Tissue Engineering Part a
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20670163
pubs.begin-page 3589
pubs.end-page 3597
pubs.issue 12
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Molecular Physiology Institute
pubs.organisational-group Duke Science & Society
pubs.organisational-group Initiatives
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group School of Nursing
pubs.organisational-group School of Nursing - Secondary Group
pubs.publication-status Published
pubs.volume 16
dc.identifier.eissn 1937-335X
duke.contributor.orcid Kraus, William E|0000-0003-1930-9684


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