Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.
Abstract
Surgery is one of the most effective and widely used procedures in treating human
cancers, but a major problem is that the surgeon often fails to remove the entire
tumor, leaving behind tumor-positive margins, metastatic lymph nodes, and/or satellite
tumor nodules. Here we report the use of a hand-held spectroscopic pen device (termed
SpectroPen) and near-infrared contrast agents for intraoperative detection of malignant
tumors, based on wavelength-resolved measurements of fluorescence and surface-enhanced
Raman scattering (SERS) signals. The SpectroPen utilizes a near-infrared diode laser
(emitting at 785 nm) coupled to a compact head unit for light excitation and collection.
This pen-shaped device effectively removes silica Raman peaks from the fiber optics
and attenuates the reflected excitation light, allowing sensitive analysis of both
fluorescence and Raman signals. Its overall performance has been evaluated by using
a fluorescent contrast agent (indocyanine green, or ICG) as well as a surface-enhanced
Raman scattering (SERS) contrast agent (pegylated colloidal gold). Under in vitro
conditions, the detection limits are approximately 2-5 × 10(-11) M for the indocyanine
dye and 0.5-1 × 10(-13) M for the SERS contrast agent. Ex vivo tissue penetration
data show attenuated but resolvable fluorescence and Raman signals when the contrast
agents are buried 5-10 mm deep in fresh animal tissues. In vivo studies using mice
bearing bioluminescent 4T1 breast tumors further demonstrate that the tumor borders
can be precisely detected preoperatively and intraoperatively, and that the contrast
signals are strongly correlated with tumor bioluminescence. After surgery, the SpectroPen
device permits further evaluation of both positive and negative tumor margins around
the surgical cavity, raising new possibilities for real-time tumor detection and image-guided
surgery.
Type
Journal articleSubject
AnimalsBreast Neoplasms
Cell Line, Tumor
Contrast Media
Equipment Design
Female
Fluorescent Dyes
Humans
Indocyanine Green
Mice
Optical Imaging
Spectrometry, Fluorescence
Spectrum Analysis, Raman
Swine
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https://hdl.handle.net/10161/3997Published Version (Please cite this version)
10.1021/ac102058kPublication Info
Mohs, AM; Mancini, MC; Singhal, S; Provenzale, JM; Leyland Jones, B; Wang, MD; & Nie,
S (2010). Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast
enhancement, detection sensitivity, and tissue penetration. Anal Chem, 82(21). pp. 9058-9065. 10.1021/ac102058k. Retrieved from https://hdl.handle.net/10161/3997.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
James Michael Provenzale
Professor of Radiology
I have the following major research areas:I. Diffusion tensor imaging (an MR technique
that measures rate and direction of microscopic water motion) to examine white matter
pathways in the brain. This technique is used by many investigators in an attempt
to understand white matter microstructure. My recent work has centered on the histological
correlation of DTI metrics. In addition, because DTI metrics can vary substantially
within a single scanner at multiple time points as well as be

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