Show simple item record Mohs, AM Mancini, MC Singhal, S Provenzale, JM Leyland-Jones, B Wang, MD Nie, S
dc.coverage.spatial United States 2011-06-21T17:22:07Z 2010-11-01
dc.identifier.citation Anal Chem, 2010, 82 (21), pp. 9058 - 9065
dc.description.abstract Surgery is one of the most effective and widely used procedures in treating human cancers, but a major problem is that the surgeon often fails to remove the entire tumor, leaving behind tumor-positive margins, metastatic lymph nodes, and/or satellite tumor nodules. Here we report the use of a hand-held spectroscopic pen device (termed SpectroPen) and near-infrared contrast agents for intraoperative detection of malignant tumors, based on wavelength-resolved measurements of fluorescence and surface-enhanced Raman scattering (SERS) signals. The SpectroPen utilizes a near-infrared diode laser (emitting at 785 nm) coupled to a compact head unit for light excitation and collection. This pen-shaped device effectively removes silica Raman peaks from the fiber optics and attenuates the reflected excitation light, allowing sensitive analysis of both fluorescence and Raman signals. Its overall performance has been evaluated by using a fluorescent contrast agent (indocyanine green, or ICG) as well as a surface-enhanced Raman scattering (SERS) contrast agent (pegylated colloidal gold). Under in vitro conditions, the detection limits are approximately 2-5 × 10(-11) M for the indocyanine dye and 0.5-1 × 10(-13) M for the SERS contrast agent. Ex vivo tissue penetration data show attenuated but resolvable fluorescence and Raman signals when the contrast agents are buried 5-10 mm deep in fresh animal tissues. In vivo studies using mice bearing bioluminescent 4T1 breast tumors further demonstrate that the tumor borders can be precisely detected preoperatively and intraoperatively, and that the contrast signals are strongly correlated with tumor bioluminescence. After surgery, the SpectroPen device permits further evaluation of both positive and negative tumor margins around the surgical cavity, raising new possibilities for real-time tumor detection and image-guided surgery.
dc.format.extent 9058 - 9065
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof Anal Chem
dc.relation.isversionof 10.1021/ac102058k
dc.subject Animals
dc.subject Breast Neoplasms
dc.subject Cell Line, Tumor
dc.subject Contrast Media
dc.subject Equipment Design
dc.subject Female
dc.subject Fluorescent Dyes
dc.subject Humans
dc.subject Indocyanine Green
dc.subject Mice
dc.subject Optical Imaging
dc.subject Spectrometry, Fluorescence
dc.subject Spectrum Analysis, Raman
dc.subject Swine
dc.title Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-11-1 en_US
duke.description.endpage 9065 en_US
duke.description.issue 21 en_US
duke.description.startpage 9058 en_US
duke.description.volume 82 en_US
dc.relation.journal Analytical Chemistry en_US
pubs.issue 21
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Duke Institute for Brain Sciences
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Radiology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Radiology/Radiology, Neuroradiology
pubs.publication-status Published
pubs.volume 82
dc.identifier.eissn 1520-6882

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