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Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.

dc.contributor.author Mohs, Aaron M
dc.contributor.author Mancini, Michael C
dc.contributor.author Singhal, Sunil
dc.contributor.author Provenzale, James M
dc.contributor.author Leyland-Jones, Brian
dc.contributor.author Wang, May D
dc.contributor.author Nie, Shuming
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:22:07Z
dc.date.issued 2010-11-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20925393
dc.identifier.uri https://hdl.handle.net/10161/3997
dc.description.abstract Surgery is one of the most effective and widely used procedures in treating human cancers, but a major problem is that the surgeon often fails to remove the entire tumor, leaving behind tumor-positive margins, metastatic lymph nodes, and/or satellite tumor nodules. Here we report the use of a hand-held spectroscopic pen device (termed SpectroPen) and near-infrared contrast agents for intraoperative detection of malignant tumors, based on wavelength-resolved measurements of fluorescence and surface-enhanced Raman scattering (SERS) signals. The SpectroPen utilizes a near-infrared diode laser (emitting at 785 nm) coupled to a compact head unit for light excitation and collection. This pen-shaped device effectively removes silica Raman peaks from the fiber optics and attenuates the reflected excitation light, allowing sensitive analysis of both fluorescence and Raman signals. Its overall performance has been evaluated by using a fluorescent contrast agent (indocyanine green, or ICG) as well as a surface-enhanced Raman scattering (SERS) contrast agent (pegylated colloidal gold). Under in vitro conditions, the detection limits are approximately 2-5 × 10(-11) M for the indocyanine dye and 0.5-1 × 10(-13) M for the SERS contrast agent. Ex vivo tissue penetration data show attenuated but resolvable fluorescence and Raman signals when the contrast agents are buried 5-10 mm deep in fresh animal tissues. In vivo studies using mice bearing bioluminescent 4T1 breast tumors further demonstrate that the tumor borders can be precisely detected preoperatively and intraoperatively, and that the contrast signals are strongly correlated with tumor bioluminescence. After surgery, the SpectroPen device permits further evaluation of both positive and negative tumor margins around the surgical cavity, raising new possibilities for real-time tumor detection and image-guided surgery.
dc.language eng
dc.language.iso en_US
dc.publisher American Chemical Society (ACS)
dc.relation.ispartof Anal Chem
dc.relation.isversionof 10.1021/ac102058k
dc.subject Animals
dc.subject Breast Neoplasms
dc.subject Cell Line, Tumor
dc.subject Contrast Media
dc.subject Equipment Design
dc.subject Female
dc.subject Fluorescent Dyes
dc.subject Humans
dc.subject Indocyanine Green
dc.subject Mice
dc.subject Optical Imaging
dc.subject Spectrometry, Fluorescence
dc.subject Spectrum Analysis, Raman
dc.subject Swine
dc.title Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.
dc.title.alternative
dc.type Journal article
duke.contributor.id Provenzale, James M|0098129
dc.description.version Version of Record
duke.date.pubdate 2010-11-1
duke.description.issue 21
duke.description.volume 82
dc.relation.journal Analytical Chemistry
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20925393
pubs.begin-page 9058
pubs.end-page 9065
pubs.issue 21
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Radiology
pubs.organisational-group Radiology, Neuroradiology
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 82
dc.identifier.eissn 1520-6882


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