Mechanical Distortion of Protein Receptor Decreases the Lifetime of a Receptor-Ligand Bond
Abstract
Substantial experimental evidence indicates that the mechanical force applied to pull
apart non-covalent molecular bonds (such as receptor ligand pairs) can significantly
decrease the bond lifetime. This evidence is often generated in single-molecule experiments
that are designed to specifically test effects of pulling forces. However, the effect
of compressive forces on the lifetime of receptor ligand bonds remains largely unexplored.
Here we extend the common usage of the atomic force microscopy technique to study
whether compressive forces applied to bound streptavidin-biotin species can significantly
accelerate the rate of dissociation. Presented experimental data indicate that compressive
forces can substantially decrease the lifetime of the molecular bond. Surprisingly,
the efficiency of accelerating dissociation by compressive forces sometimes exceeds
the enhancement of the dissociation rate measured in pulling experiments, indicating
that compressive forces applied to the bound species might be efficiently used to
control the lifetime of adhesion bonds.
Type
Other articleSubject
force spectroscopy measurementsatomic-force
molecular recognition
microscopy
afm
surface
cell
chemistry
kinetics
ruptures
chemistry, multidisciplinary
Permalink
https://hdl.handle.net/10161/4041Published Version (Please cite this version)
10.1021/ja1011756Citation
Guo,Senli;Li,Nan;Lad,Nimit;Ray,Chad;Akhremitchev,Boris B.. 2010. Mechanical Distortion
of Protein Receptor Decreases the Lifetime of a Receptor-Ligand Bond. Journal of the
American Chemical Society 132(28): 9681-9687.
Collections
More Info
Show full item record
Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info