Separating DNA with different topologies by atomic force microscopy in comparison with gel electrophoresis.
Repository Usage Stats
Atomic force microscopy, which is normally used for DNA imaging to gain qualitative results, can also be used for quantitative DNA research, at a single-molecular level. Here, we evaluate the performance of AFM imaging specifically for quantifying supercoiled and relaxed plasmid DNA fractions within a mixture, and compare the results with the bulk material analysis method, gel electrophoresis. The advantages and shortcomings of both methods are discussed in detail. Gel electrophoresis is a quick and well-established quantification method. However, it requires a large amount of DNA, and needs to be carefully calibrated for even slightly different experimental conditions for accurate quantification. AFM imaging is accurate, in that single DNA molecules in different conformations can be seen and counted. When used carefully with necessary correction, both methods provide consistent results. Thus, AFM imaging can be used for DNA quantification, as an alternative to gel electrophoresis.
Published Version (Please cite this version)10.1021/jp105603k
Publication InfoJiang, Yong; Rabbi, Mahir; Mieczkowski, Piotr A; & Marszalek, Piotr E (2010). Separating DNA with different topologies by atomic force microscopy in comparison with gel electrophoresis. J Phys Chem B, 114(37). pp. 12162-12165. 10.1021/jp105603k. Retrieved from https://hdl.handle.net/10161/4074.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
More InfoShow full item record
Professor of Mechanical Engineering and Materials Science
My research focuses on investigating relationships between structural and mechanical properties of biopolymers (polysaccharides, DNA, proteins), which I study at a single molecule level. My main approaches are experimental scanning probe microscopy techniques and computational methods involving Molecular Dynamics simulations and ab initio quantum mechanical calculations. The ultimate goal of this research is to understand the above-mentioned relationships at an atomic level and to apply the know