Deformation of stem cell nuclei by nanotopographical cues.
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Cells sense cues in their surrounding microenvironment. These cues are converted into intracellular signals and transduced to the nucleus in order for the cell to respond and adapt its function. Within the nucleus, structural changes occur that ultimately lead to changes in the gene expression. In this study, we explore the structural changes of the nucleus of human mesenchymal stem cells as an effect of topographical cues. We use a controlled nanotopography to drive shape changes to the cell nucleus, and measure the changes with both fluorescence microscopy and a novel light scattering technique. The nucleus changes shape dramatically in response to the nanotopography, and in a manner dependent on the mechanical properties of the substrate. The kinetics of the nuclear deformation follows an unexpected trajectory. As opposed to a gradual shape change in response to the topography, once the cytoskeleton attains an aligned and elongation morphology on the time scale of several hours, the nucleus changes shape rapidly and intensely.
Published Version (Please cite this version)10.1039/B921206J
Publication InfoChalut, KJ; Giacomelli, Michael G; Kulangara, K; Leong, KW; & Wax, A (2010). Deformation of stem cell nuclei by nanotopographical cues. Soft Matter, 6(8). pp. 1675-1681. 10.1039/B921206J. Retrieved from http://hdl.handle.net/10161/4120.
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Professor of Biomedical Engineering
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and interferometry techniques. The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based