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Deformation of stem cell nuclei by nanotopographical cues.

dc.contributor.author Chalut, KJ
dc.contributor.author Giacomelli, Michael G
dc.contributor.author Kulangara, K
dc.contributor.author Leong, KW
dc.contributor.author Wax, A
dc.coverage.spatial England
dc.date.accessioned 2011-06-21T17:27:13Z
dc.date.issued 2010-04-21
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/21297875
dc.identifier.uri http://hdl.handle.net/10161/4120
dc.description.abstract Cells sense cues in their surrounding microenvironment. These cues are converted into intracellular signals and transduced to the nucleus in order for the cell to respond and adapt its function. Within the nucleus, structural changes occur that ultimately lead to changes in the gene expression. In this study, we explore the structural changes of the nucleus of human mesenchymal stem cells as an effect of topographical cues. We use a controlled nanotopography to drive shape changes to the cell nucleus, and measure the changes with both fluorescence microscopy and a novel light scattering technique. The nucleus changes shape dramatically in response to the nanotopography, and in a manner dependent on the mechanical properties of the substrate. The kinetics of the nuclear deformation follows an unexpected trajectory. As opposed to a gradual shape change in response to the topography, once the cytoskeleton attains an aligned and elongation morphology on the time scale of several hours, the nucleus changes shape rapidly and intensely.
dc.language eng
dc.language.iso en_US
dc.relation.ispartof Soft Matter
dc.relation.isversionof 10.1039/B921206J
dc.title Deformation of stem cell nuclei by nanotopographical cues.
dc.title.alternative
dc.type Journal article
dc.description.version Version of Record
duke.date.pubdate 2010-00-00
duke.description.issue 8
duke.description.volume 6
dc.relation.journal Soft Matter
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/21297875
pubs.begin-page 1675
pubs.end-page 1681
pubs.issue 8
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 6
dc.identifier.eissn 1744-6848


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