Show simple item record Chalut, KJ Kulangara, K Giacomelli, MG Wax, A Leong, KW
dc.coverage.spatial England 2011-06-21T17:27:13Z 2010-04-21
dc.identifier.citation Soft Matter, 2010, 6 (8), pp. 1675 - 1681
dc.description.abstract Cells sense cues in their surrounding microenvironment. These cues are converted into intracellular signals and transduced to the nucleus in order for the cell to respond and adapt its function. Within the nucleus, structural changes occur that ultimately lead to changes in the gene expression. In this study, we explore the structural changes of the nucleus of human mesenchymal stem cells as an effect of topographical cues. We use a controlled nanotopography to drive shape changes to the cell nucleus, and measure the changes with both fluorescence microscopy and a novel light scattering technique. The nucleus changes shape dramatically in response to the nanotopography, and in a manner dependent on the mechanical properties of the substrate. The kinetics of the nuclear deformation follows an unexpected trajectory. As opposed to a gradual shape change in response to the topography, once the cytoskeleton attains an aligned and elongation morphology on the time scale of several hours, the nucleus changes shape rapidly and intensely.
dc.format.extent 1675 - 1681
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof Soft Matter
dc.relation.isversionof 10.1039/B921206J
dc.title Deformation of stem cell nuclei by nanotopographical cues.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-00-00 en_US
duke.description.endpage 1681 en_US
duke.description.issue 8 en_US
duke.description.startpage 1675 en_US
duke.description.volume 6 en_US
dc.relation.journal Soft Matter en_US
pubs.issue 8
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Duke Institute for Brain Sciences
pubs.organisational-group /Duke/Pratt School of Engineering
pubs.organisational-group /Duke/Pratt School of Engineering/Biomedical Engineering
pubs.publication-status Published
pubs.volume 6
dc.identifier.eissn 1744-6848

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