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Use of an insulating mask for controlling anisotropy in multilayer electrospun scaffolds for tissue engineering.

dc.contributor.author Garrigues, N William
dc.contributor.author Little, Dianne
dc.contributor.author O'Conor, Christopher J
dc.contributor.author Guilak, Farshid
dc.coverage.spatial England
dc.date.accessioned 2011-06-21T17:27:17Z
dc.date.issued 2010-10-28
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/21072247
dc.identifier.issn 0959-9428
dc.identifier.uri https://hdl.handle.net/10161/4133
dc.description.abstract Tissue engineering of various musculoskeletal or cardiovascular tissues requires scaffolds with controllable mechanical anisotropy. However, native tissues also exhibit significant inhomogeneity in their mechanical properties, and the principal axes of anisotropy may vary with site or depth from the tissue surface. Thus, techniques to produce multilayered biomaterial scaffolds with controllable anisotropy may provide improved biomimetic properties for functional tissue replacements. In this study, poly(ε-caprolactone) scaffolds were electrospun onto a collecting electrode that was partially covered by rectangular or square shaped insulating masks. The use of a rectangular mask resulted in aligned scaffolds that were significantly stiffer in tension in the axial direction than the transverse direction at 0 strain (22.9 ± 1.3 MPa axial, 16.1 ± 0.9 MPa transverse), and at 0.1 strain (4.8 ± 0.3 MPa axial, 3.5 ± 0.2 MPa transverse). The unaligned scaffolds, produced using a square mask, did not show this anisotropy, with similar stiffness in the axial and transverse directions at 0 strain (19.7 ± 1.4 MPa axial, 20.8 ± 1.3 MPa transverse) and 0.1 strain (4.4 ± 0.2 MPa axial, 4.6 ± 0.3 MPa, transverse). Aligned scaffolds also induced alignment of adipose stem cells near the expected axis on aligned scaffolds (0.015 ± 0.056 rad), while on the unaligned scaffolds, their orientation showed more variation and was not along the expected axis (1.005 ± 0.225 rad). This method provides a novel means of creating multilayered electrospun scaffolds with controlled anisotropy for each layer, potentially providing a means to mimic the complex mechanical properties of various native tissues.
dc.language eng
dc.language.iso en_US
dc.publisher Royal Society of Chemistry (RSC)
dc.relation.ispartof J Mater Chem
dc.relation.isversionof 10.1039/c0jm01880e
dc.title Use of an insulating mask for controlling anisotropy in multilayer electrospun scaffolds for tissue engineering.
dc.title.alternative
dc.type Journal article
duke.contributor.id Little, Dianne|0461205
duke.contributor.id Guilak, Farshid|0115558
dc.description.version Version of Record
duke.date.pubdate 2010-00-00
duke.description.issue 40
duke.description.volume 20
dc.relation.journal Journal of Materials Chemistry
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/21072247
pubs.begin-page 8962
pubs.end-page 8968
pubs.issue 40
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Duke
pubs.organisational-group Faculty
pubs.organisational-group Pratt School of Engineering
pubs.publication-status Published
pubs.volume 20


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