dc.contributor.author |
Garrigues, N William |
|
dc.contributor.author |
Little, Dianne |
|
dc.contributor.author |
O'Conor, Christopher J |
|
dc.contributor.author |
Guilak, Farshid |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2011-06-21T17:27:17Z |
|
dc.date.issued |
2010-10-28 |
|
dc.identifier |
http://www.ncbi.nlm.nih.gov/pubmed/21072247 |
|
dc.identifier.issn |
0959-9428 |
|
dc.identifier.uri |
https://hdl.handle.net/10161/4133 |
|
dc.description.abstract |
Tissue engineering of various musculoskeletal or cardiovascular tissues requires scaffolds
with controllable mechanical anisotropy. However, native tissues also exhibit significant
inhomogeneity in their mechanical properties, and the principal axes of anisotropy
may vary with site or depth from the tissue surface. Thus, techniques to produce multilayered
biomaterial scaffolds with controllable anisotropy may provide improved biomimetic
properties for functional tissue replacements. In this study, poly(ε-caprolactone)
scaffolds were electrospun onto a collecting electrode that was partially covered
by rectangular or square shaped insulating masks. The use of a rectangular mask resulted
in aligned scaffolds that were significantly stiffer in tension in the axial direction
than the transverse direction at 0 strain (22.9 ± 1.3 MPa axial, 16.1 ± 0.9 MPa transverse),
and at 0.1 strain (4.8 ± 0.3 MPa axial, 3.5 ± 0.2 MPa transverse). The unaligned scaffolds,
produced using a square mask, did not show this anisotropy, with similar stiffness
in the axial and transverse directions at 0 strain (19.7 ± 1.4 MPa axial, 20.8 ± 1.3
MPa transverse) and 0.1 strain (4.4 ± 0.2 MPa axial, 4.6 ± 0.3 MPa, transverse). Aligned
scaffolds also induced alignment of adipose stem cells near the expected axis on aligned
scaffolds (0.015 ± 0.056 rad), while on the unaligned scaffolds, their orientation
showed more variation and was not along the expected axis (1.005 ± 0.225 rad). This
method provides a novel means of creating multilayered electrospun scaffolds with
controlled anisotropy for each layer, potentially providing a means to mimic the complex
mechanical properties of various native tissues.
|
|
dc.language |
eng |
|
dc.language.iso |
en_US |
|
dc.publisher |
Royal Society of Chemistry (RSC) |
|
dc.relation.ispartof |
J Mater Chem |
|
dc.relation.isversionof |
10.1039/c0jm01880e |
|
dc.title |
Use of an insulating mask for controlling anisotropy in multilayer electrospun scaffolds
for tissue engineering.
|
|
dc.title.alternative |
|
|
dc.type |
Journal article |
|
duke.contributor.id |
Little, Dianne|0461205 |
|
duke.contributor.id |
Guilak, Farshid|0115558 |
|
dc.description.version |
Version of Record |
|
duke.date.pubdate |
2010-00-00 |
|
duke.description.issue |
40 |
|
duke.description.volume |
20 |
|
dc.relation.journal |
Journal of Materials Chemistry |
|
pubs.author-url |
http://www.ncbi.nlm.nih.gov/pubmed/21072247 |
|
pubs.begin-page |
8962 |
|
pubs.end-page |
8968 |
|
pubs.issue |
40 |
|
pubs.organisational-group |
Biomedical Engineering |
|
pubs.organisational-group |
Duke |
|
pubs.organisational-group |
Faculty |
|
pubs.organisational-group |
Pratt School of Engineering |
|
pubs.publication-status |
Published |
|
pubs.volume |
20 |
|