Show simple item record Garrigues, NW Little, D O'Conor, CJ Guilak, F
dc.coverage.spatial England 2011-06-21T17:27:17Z 2010-10-28
dc.identifier.citation J Mater Chem, 2010, 20 (40), pp. 8962 - 8968
dc.identifier.issn 0959-9428
dc.description.abstract Tissue engineering of various musculoskeletal or cardiovascular tissues requires scaffolds with controllable mechanical anisotropy. However, native tissues also exhibit significant inhomogeneity in their mechanical properties, and the principal axes of anisotropy may vary with site or depth from the tissue surface. Thus, techniques to produce multilayered biomaterial scaffolds with controllable anisotropy may provide improved biomimetic properties for functional tissue replacements. In this study, poly(ε-caprolactone) scaffolds were electrospun onto a collecting electrode that was partially covered by rectangular or square shaped insulating masks. The use of a rectangular mask resulted in aligned scaffolds that were significantly stiffer in tension in the axial direction than the transverse direction at 0 strain (22.9 ± 1.3 MPa axial, 16.1 ± 0.9 MPa transverse), and at 0.1 strain (4.8 ± 0.3 MPa axial, 3.5 ± 0.2 MPa transverse). The unaligned scaffolds, produced using a square mask, did not show this anisotropy, with similar stiffness in the axial and transverse directions at 0 strain (19.7 ± 1.4 MPa axial, 20.8 ± 1.3 MPa transverse) and 0.1 strain (4.4 ± 0.2 MPa axial, 4.6 ± 0.3 MPa, transverse). Aligned scaffolds also induced alignment of adipose stem cells near the expected axis on aligned scaffolds (0.015 ± 0.056 rad), while on the unaligned scaffolds, their orientation showed more variation and was not along the expected axis (1.005 ± 0.225 rad). This method provides a novel means of creating multilayered electrospun scaffolds with controlled anisotropy for each layer, potentially providing a means to mimic the complex mechanical properties of various native tissues.
dc.format.extent 8962 - 8968
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof J Mater Chem
dc.relation.isversionof 10.1039/c0jm01880e
dc.title Use of an insulating mask for controlling anisotropy in multilayer electrospun scaffolds for tissue engineering.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-00-00 en_US
duke.description.endpage 8968 en_US
duke.description.issue 40 en_US
duke.description.startpage 8962 en_US
duke.description.volume 20 en_US
dc.relation.journal Journal of Materials Chemistry en_US
pubs.issue 40
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Faculty
pubs.organisational-group /Duke/Pratt School of Engineering
pubs.organisational-group /Duke/Pratt School of Engineering/Biomedical Engineering
pubs.publication-status Published
pubs.volume 20

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