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Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi.

dc.contributor.author Idnurm, Alexander
dc.contributor.author Heitman, Joseph
dc.coverage.spatial England
dc.date.accessioned 2011-06-21T17:27:29Z
dc.date.issued 2010-08
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20488877
dc.identifier mic.0.039222-0
dc.identifier.uri https://hdl.handle.net/10161/4166
dc.description.abstract Light is a universal signal perceived by organisms, including fungi, in which light regulates common and unique biological processes depending on the species. Previous research has established that conserved proteins, originally called White collar 1 and 2 from the ascomycete Neurospora crassa, regulate UV/blue light sensing. Homologous proteins function in distant relatives of N. crassa, including the basidiomycetes and zygomycetes, which diverged as long as a billion years ago. Here we conducted microarray experiments on the basidiomycete fungus Cryptococcus neoformans to identify light-regulated genes. Surprisingly, only a single gene was induced by light above the commonly used twofold threshold. This gene, HEM15, is predicted to encode a ferrochelatase that catalyses the final step in haem biosynthesis from highly photoreactive porphyrins. The C. neoformans gene complements a Saccharomyces cerevisiae hem15Delta strain and is essential for viability, and the Hem15 protein localizes to mitochondria, three lines of evidence that the gene encodes ferrochelatase. Regulation of HEM15 by light suggests a mechanism by which bwc1/bwc2 mutants are photosensitive and exhibit reduced virulence. We show that ferrochelatase is also light-regulated in a white collar-dependent fashion in N. crassa and the zygomycete Phycomyces blakesleeanus, indicating that ferrochelatase is an ancient target of photoregulation in the fungal kingdom.
dc.language eng
dc.language.iso en_US
dc.publisher Microbiology Society
dc.relation.ispartof Microbiology
dc.relation.isversionof 10.1099/mic.0.039222-0
dc.subject Cryptococcus neoformans
dc.subject Ferrochelatase
dc.subject Fungal Proteins
dc.subject Gene Deletion
dc.subject Gene Expression Regulation, Fungal
dc.subject Genes, Fungal
dc.subject Genetic Complementation Test
dc.subject Light
dc.subject Mitochondria
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject Phycomyces
dc.subject RNA, Fungal
dc.subject Saccharomyces cerevisiae
dc.title Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi.
dc.title.alternative
dc.type Journal article
duke.contributor.id Heitman, Joseph|0113253
dc.description.version Version of Record
duke.date.pubdate 2010-8-0
duke.description.issue
duke.description.volume 156
dc.relation.journal Microbiology-Sgm
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20488877
pubs.begin-page 2393
pubs.end-page 2407
pubs.issue Pt 8
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Infectious Diseases
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Pharmacology & Cancer Biology
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 156
dc.identifier.eissn 1465-2080


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