BMP signaling in the development of the mouse esophagus and forestomach.
Abstract
The stratification and differentiation of the epidermis are known to involve the precise
control of multiple signaling pathways. By contrast, little is known about the development
of the mouse esophagus and forestomach, which are composed of a stratified squamous
epithelium. Based on prior work in the skin, we hypothesized that bone morphogenetic
protein (BMP) signaling is a central player. To test this hypothesis, we first used
a BMP reporter mouse line harboring a BRE-lacZ allele, along with in situ hybridization
to localize transcripts for BMP signaling components, including various antagonists.
We then exploited a Shh-Cre allele that drives recombination in the embryonic foregut
epithelium to generate gain- or loss-of-function models for the Bmpr1a (Alk3) receptor.
In gain-of-function (Shh-Cre;Rosa26(CAG-loxpstoploxp-caBmprIa)) embryos, high levels
of ectopic BMP signaling stall the transition from simple columnar to multilayered
undifferentiated epithelium in the esophagus and forestomach. In loss-of-function
experiments, conditional deletion of the BMP receptor in Shh-Cre;Bmpr1a(flox/flox)
embryos allows the formation of a multilayered squamous epithelium but this fails
to differentiate, as shown by the absence of expression of the suprabasal markers
loricrin and involucrin. Together, these findings suggest multiple roles for BMP signaling
in the developing esophagus and forestomach.
Type
Journal articleSubject
AnimalsBone Morphogenetic Protein Receptors
Bone Morphogenetic Protein Receptors, Type I
Bone Morphogenetic Proteins
Carrier Proteins
Epithelium
Esophagus
Gene Expression Regulation, Developmental
Hedgehog Proteins
Immunohistochemistry
In Situ Hybridization
Mice
Signal Transduction
Stomach
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https://hdl.handle.net/10161/4178Published Version (Please cite this version)
10.1242/dev.056077Publication Info
Rodriguez, Pavel; Da Silva, Susana; Oxburgh, Leif; Wang, Fan; Hogan, Brigid LM; &
Que, Jianwen (2010). BMP signaling in the development of the mouse esophagus and forestomach. Development, 137(24). pp. 4171-4176. 10.1242/dev.056077. Retrieved from https://hdl.handle.net/10161/4178.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Brigid L. M. Hogan
Research Professor of Cell Biology
1. Genetic regulation of embryo development using the mouse as a research model. 2.
The role of genes and signaling pathways in directing and co-ordinating the development
of the lung. 3. The identity and regulation of the different stem cells in the adult
lung and their role in repair, fibrosis and cancer.
Fan Wang
Adjunct Professor in the Department of Neurobiology
My lab studies neural circuit basis of sensory perception. Specifically we are interested
in determining neural circuits underlying (1) active touch sensation including tactile
processing stream and motor control of touch sensors on the face; (2) pain sensation
including both sensory-discriminative and affective aspects of pain; and (3) general
anesthesia including the active pain-suppression process. We use a combination of
genetic, viral, electrophysiology, and in vivo imaging (in f
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