Show simple item record Lee, Y Nachtrab, G Klinsawat, PW Hami, D Poss, KD
dc.coverage.spatial England 2011-06-21T17:27:32Z 2010-07
dc.identifier dmm.004515
dc.identifier.citation Dis Model Mech, 2010, 3 (7-8), pp. 496 - 503
dc.description.abstract Regenerative medicine for complex tissues like limbs will require the provision or activation of precursors for different cell types, in the correct number, and with the appropriate instructions. These strategies can be guided by what is learned from spectacular events of natural limb or fin regeneration in urodele amphibians and teleost fish. Following zebrafish fin amputation, melanocyte stripes faithfully regenerate in tandem with complex fin structures. Distinct populations of melanocyte precursors emerge and differentiate to pigment regenerating fins, yet the regulation of their proliferation and patterning is incompletely understood. Here, we found that transgenic increases in active Ras dose-dependently hyperpigmented regenerating zebrafish fins. Lineage tracing and marker analysis indicated that increases in active Ras stimulated the in situ amplification of undifferentiated melanocyte precursors expressing mitfa and kita. Active Ras also hyperpigmented early fin regenerates of kita mutants, which are normally devoid of primary regeneration melanocytes, suppressing defects in precursor function and survival. By contrast, this protocol had no noticeable impact on pigmentation by secondary regulatory melanocyte precursors in late-stage kita regenerates. Our results provide evidence that Ras activity levels control the repopulation and expansion of adult melanocyte precursors after tissue loss, enabling the recovery of patterned melanocyte stripes during zebrafish appendage regeneration.
dc.format.extent 496 - 503
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof Dis Model Mech
dc.relation.isversionof 10.1242/dmm.004515
dc.subject Animal Structures
dc.subject Animals
dc.subject Animals, Genetically Modified
dc.subject Cell Differentiation
dc.subject Cell Proliferation
dc.subject Melanocytes
dc.subject Pigmentation
dc.subject Regeneration
dc.subject Stem Cells
dc.subject Zebrafish
dc.subject Zebrafish Proteins
dc.subject ras Proteins
dc.title Ras controls melanocyte expansion during zebrafish fin stripe regeneration.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-8-jul en_US
duke.description.endpage 503 en_US
duke.description.issue 8-Jul en_US
duke.description.startpage 496 en_US
duke.description.volume 3 en_US
dc.relation.journal Disease Models & Mechanisms en_US
pubs.issue 7-8
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Cell Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Cardiology
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.organisational-group /Duke/Trinity College of Arts & Sciences
pubs.organisational-group /Duke/Trinity College of Arts & Sciences/Biology
pubs.publication-status Published
pubs.volume 3
dc.identifier.eissn 1754-8411

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