Changes in Bni4 localization induced by cell stress in Saccharomyces cerevisiae
Abstract
Septin complexes at the bud neck in Saccharomyces cerevisiae serve as a scaffold for
proteins involved in signaling, cell cycle control, and cell wall synthesis. Many
of these bind asymmetrically, associating with either the mother-or daughter-side
of the neck. Septin structures are inherently apolar so the basis for the asymmetric
binding remains unknown. Bni4, a regulatory subunit of yeast protein phosphatase type
1, Glc7, binds to the outside of the septin ring prior to bud formation and remains
restricted to the mother-side of the bud neck after bud emergence. Bni4 is responsible
for targeting Glc7 to the mother-side of the bud neck for proper deposition of the
chitin ring. We show here that Bni4 localizes symmetrically, as two distinct rings
on both sides of the bud neck following energy depletion or activation of cell cycle
checkpoints. Our data indicate that loss of Bni4 asymmetry can occur via at least
two different mechanisms. Furthermore, we show that Bni4 has a Swe1-dependent role
in regulating the cell morphogenesis checkpoint in response to hydroxyurea, which
suggests that the change in localization of Bni4 following checkpoint activation may
help stabilize the cell cycle regulator Swe1 during cell cycle arrest.
Type
Other articleSubject
bni4glc7
cell stress
septins
protein phosphatase type-1
septin organization
budding yeast
interaction network
binding protein
bud neck
cycle
gene
ring
compartmentalization
cell biology
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https://hdl.handle.net/10161/4188Published Version (Please cite this version)
10.1242/jcs.066258Citation
Larson,Jennifer R.;Kozubowski,Lukasz;Tatchell,Kelly. 2010. Changes in Bni4 localization
induced by cell stress in Saccharomyces cerevisiae. Journal of cell science 123(7):
1050-1059.
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