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Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.

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Date
2010-07-01
Authors
Jones, Whitney M
Chao, Anna T
Zavortink, Michael
Saint, Robert
Bejsovec, Amy
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Abstract
Wg/Wnt signals specify cell fates in both invertebrate and vertebrate embryos and maintain stem-cell populations in many adult tissues. Deregulation of the Wnt pathway can transform cells to a proliferative fate, leading to cancer. We have discovered that two Drosophila proteins that are crucial for cytokinesis have a second, largely independent, role in restricting activity of the Wnt pathway. The fly homolog of RacGAP1, Tumbleweed (Tum)/RacGAP50C, and its binding partner, the kinesin-like protein Pavarotti (Pav), negatively regulate Wnt activity in fly embryos and in cultured mammalian cells. Unlike many known regulators of the Wnt pathway, these molecules do not affect stabilization of Arm/beta-catenin (betacat), the principal effector molecule in Wnt signal transduction. Rather, they appear to act downstream of betacat stabilization to control target-gene transcription. Both Tum and Pav accumulate in the nuclei of interphase cells, a location that is spatially distinct from their cleavage-furrow localization during cytokinesis. We show that this nuclear localization is essential for their role in Wnt regulation. Thus, we have identified two modulators of the Wnt pathway that have shared functions in cell division, which hints at a possible link between cytokinesis and Wnt activity during tumorigenesis.
Type
Journal article
Subject
Animals
Armadillo Domain Proteins
Body Patterning
Cell Nucleus
Cells, Cultured
Cytokinesis
Drosophila Proteins
Drosophila melanogaster
Epistasis, Genetic
GTPase-Activating Proteins
Genes, Reporter
Humans
Microtubule-Associated Proteins
Phenotype
Signal Transduction
Transcription Factors
Two-Hybrid System Techniques
Wings, Animal
Wnt Proteins
Wnt1 Protein
beta Catenin
Permalink
https://hdl.handle.net/10161/4189
Published Version (Please cite this version)
10.1242/jcs.067868
Publication Info
Jones, Whitney M; Chao, Anna T; Zavortink, Michael; Saint, Robert; & Bejsovec, Amy (2010). Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation. J Cell Sci, 123(Pt 13). pp. 2179-2189. 10.1242/jcs.067868. Retrieved from https://hdl.handle.net/10161/4189.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Bejsovec

Amy Bejsovec

Associate Professor of Biology
My laboratory explores the molecular mechanisms of pattern formation in developing embryos. We focus on the Wingless(Wg)/Wnt class of secreted growth factor: these molecules promote cell-cell communication leading to important cell fate decisions during the development of both vertebrate and invertebrate embryos. In addition, this highly conserved pathway is essential for maintaining stem cell populations and is associated with human cancers when inappropriately activated in adult tissues. Wg/Wn
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