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Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.

dc.contributor.author Bejsovec, Amy
dc.contributor.author Chao, AT
dc.contributor.author Jones, WM
dc.contributor.author Saint, R
dc.contributor.author Zavortink, M
dc.coverage.spatial England
dc.date.accessioned 2011-06-21T17:27:36Z
dc.date.issued 2010-07-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20516152
dc.identifier jcs.067868
dc.identifier.uri http://hdl.handle.net/10161/4189
dc.description.abstract Wg/Wnt signals specify cell fates in both invertebrate and vertebrate embryos and maintain stem-cell populations in many adult tissues. Deregulation of the Wnt pathway can transform cells to a proliferative fate, leading to cancer. We have discovered that two Drosophila proteins that are crucial for cytokinesis have a second, largely independent, role in restricting activity of the Wnt pathway. The fly homolog of RacGAP1, Tumbleweed (Tum)/RacGAP50C, and its binding partner, the kinesin-like protein Pavarotti (Pav), negatively regulate Wnt activity in fly embryos and in cultured mammalian cells. Unlike many known regulators of the Wnt pathway, these molecules do not affect stabilization of Arm/beta-catenin (betacat), the principal effector molecule in Wnt signal transduction. Rather, they appear to act downstream of betacat stabilization to control target-gene transcription. Both Tum and Pav accumulate in the nuclei of interphase cells, a location that is spatially distinct from their cleavage-furrow localization during cytokinesis. We show that this nuclear localization is essential for their role in Wnt regulation. Thus, we have identified two modulators of the Wnt pathway that have shared functions in cell division, which hints at a possible link between cytokinesis and Wnt activity during tumorigenesis.
dc.language eng
dc.language.iso en_US
dc.relation.ispartof J Cell Sci
dc.relation.isversionof 10.1242/jcs.067868
dc.subject Animals
dc.subject Armadillo Domain Proteins
dc.subject Body Patterning
dc.subject Cell Nucleus
dc.subject Cells, Cultured
dc.subject Cytokinesis
dc.subject Drosophila Proteins
dc.subject Drosophila melanogaster
dc.subject Epistasis, Genetic
dc.subject GTPase-Activating Proteins
dc.subject Genes, Reporter
dc.subject Humans
dc.subject Microtubule-Associated Proteins
dc.subject Phenotype
dc.subject Signal Transduction
dc.subject Transcription Factors
dc.subject Two-Hybrid System Techniques
dc.subject Wings, Animal
dc.subject Wnt Proteins
dc.subject Wnt1 Protein
dc.subject beta Catenin
dc.title Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.
dc.title.alternative
dc.type Journal article
dc.description.version Version of Record
duke.date.pubdate 2010-7-1
duke.description.issue 13
duke.description.volume 123
dc.relation.journal Journal of cell science
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20516152
pubs.begin-page 2179
pubs.end-page 2189
pubs.issue Pt 13
pubs.organisational-group Biology
pubs.organisational-group Duke
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 123
dc.identifier.eissn 1477-9137


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