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    Facilitative glucose transporter Glut1 is actively excluded from rod outer segments.

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    Date
    2010-11-01
    Authors
    Arshavsky, Vadim
    Baker, SA
    Gospe, SM
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    Abstract
    Photoreceptors are among the most metabolically active cells in the body, relying on both oxidative phosphorylation and glycolysis to satisfy their high energy needs. Local glycolysis is thought to be particularly crucial in supporting the function of the photoreceptor's light-sensitive outer segment compartment, which is devoid of mitochondria. Accordingly, it has been commonly accepted that the facilitative glucose transporter Glut1 responsible for glucose entry into photoreceptors is localized in part to the outer segment plasma membrane. However, we now demonstrate that Glut1 is entirely absent from the rod outer segment and is actively excluded from this compartment by targeting information present in its cytosolic C-terminal tail. Our data indicate that glucose metabolized in the outer segment must first enter through other parts of the photoreceptor cell. Consequently, the entire energy supply of the outer segment is dependent on diffusion of energy-rich substrates through the thin connecting cilium that links this compartment to the rest of the cell.
    Type
    Journal article
    Subject
    Animals
    Animals, Genetically Modified
    Facilitated Diffusion
    Glucose
    Glucose Transporter Type 1
    Mice
    Mice, Inbred Strains
    Mutagenesis, Site-Directed
    Photoreceptor Connecting Cilium
    Protein Engineering
    Protein Sorting Signals
    Protein Transport
    Rats
    Retinal Photoreceptor Cell Inner Segment
    Rod Cell Outer Segment
    Sequence Deletion
    Transgenes
    Xenopus laevis
    Permalink
    https://hdl.handle.net/10161/4190
    Published Version (Please cite this version)
    10.1242/jcs.072389
    Publication Info
    Arshavsky, Vadim; Baker, SA; & Gospe, SM (2010). Facilitative glucose transporter Glut1 is actively excluded from rod outer segments. J Cell Sci, 123(Pt 21). pp. 3639-3644. 10.1242/jcs.072389. Retrieved from https://hdl.handle.net/10161/4190.
    This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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    Scholars@Duke

    Arshavsky

    Vadim Y Arshavsky

    Helena Rubinstein Foundation Professor of Ophthalmology
    Research conducted in our laboratory is dedicated to understanding how vision is performed on the molecular level. Our most mature direction addresses the function of rod and cone photoreceptors, which are sensory neurons responsible for the detection and primary processing of information entering the eye in the form of photons. Photoreceptors respond to capturing photons by generating electrical signals transmitted to the secondary neurons in the retina and, ultimately, to the brain. Our wor
    Open Access

    Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy

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