Show simple item record Gospe, SM Baker, SA Arshavsky, VY
dc.coverage.spatial England 2011-06-21T17:27:36Z 2010-11-01
dc.identifier jcs.072389
dc.identifier.citation J Cell Sci, 2010, 123 (Pt 21), pp. 3639 - 3644
dc.description.abstract Photoreceptors are among the most metabolically active cells in the body, relying on both oxidative phosphorylation and glycolysis to satisfy their high energy needs. Local glycolysis is thought to be particularly crucial in supporting the function of the photoreceptor's light-sensitive outer segment compartment, which is devoid of mitochondria. Accordingly, it has been commonly accepted that the facilitative glucose transporter Glut1 responsible for glucose entry into photoreceptors is localized in part to the outer segment plasma membrane. However, we now demonstrate that Glut1 is entirely absent from the rod outer segment and is actively excluded from this compartment by targeting information present in its cytosolic C-terminal tail. Our data indicate that glucose metabolized in the outer segment must first enter through other parts of the photoreceptor cell. Consequently, the entire energy supply of the outer segment is dependent on diffusion of energy-rich substrates through the thin connecting cilium that links this compartment to the rest of the cell.
dc.format.extent 3639 - 3644
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof J Cell Sci
dc.relation.isversionof 10.1242/jcs.072389
dc.subject Animals
dc.subject Animals, Genetically Modified
dc.subject Facilitated Diffusion
dc.subject Glucose
dc.subject Glucose Transporter Type 1
dc.subject Mice
dc.subject Mice, Inbred Strains
dc.subject Mutagenesis, Site-Directed
dc.subject Photoreceptor Connecting Cilium
dc.subject Protein Engineering
dc.subject Protein Sorting Signals
dc.subject Protein Transport
dc.subject Rats
dc.subject Retinal Photoreceptor Cell Inner Segment
dc.subject Rod Cell Outer Segment
dc.subject Sequence Deletion
dc.subject Transgenes
dc.subject Xenopus laevis
dc.title Facilitative glucose transporter Glut1 is actively excluded from rod outer segments.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-11-1 en_US
duke.description.endpage 3644 en_US
duke.description.issue 21 en_US
duke.description.startpage 3639 en_US
duke.description.volume 123 en_US
dc.relation.journal Journal of cell science en_US
pubs.issue Pt 21
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Duke Institute for Brain Sciences
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Ophthalmology
pubs.publication-status Published
pubs.volume 123
dc.identifier.eissn 1477-9137

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