Show simple item record Williams, LE Wernegreen, JJ
dc.coverage.spatial England 2011-06-21T17:29:34Z 2010-12-02
dc.identifier 1471-2164-11-687
dc.identifier.citation BMC Genomics, 2010, 11 pp. 687 - ?
dc.description.abstract BACKGROUND: Blochmannia are obligately intracellular bacterial mutualists of ants of the tribe Camponotini. Blochmannia perform key nutritional functions for the host, including synthesis of several essential amino acids. We used Illumina technology to sequence the genome of Blochmannia associated with Camponotus vafer. RESULTS: Although Blochmannia vafer retains many nutritional functions, it is missing glutamine synthetase (glnA), a component of the nitrogen recycling pathway encoded by the previously sequenced B. floridanus and B. pennsylvanicus. With the exception of Ureaplasma, B. vafer is the only sequenced bacterium to date that encodes urease but lacks the ability to assimilate ammonia into glutamine or glutamate. Loss of glnA occurred in a deletion hotspot near the putative replication origin. Overall, compared to the likely gene set of their common ancestor, 31 genes are missing or eroded in B. vafer, compared to 28 in B. floridanus and four in B. pennsylvanicus. Three genes (queA, visC and yggS) show convergent loss or erosion, suggesting relaxed selection for their functions. Eight B. vafer genes contain frameshifts in homopolymeric tracts that may be corrected by transcriptional slippage. Two of these encode DNA replication proteins: dnaX, which we infer is also frameshifted in B. floridanus, and dnaG. CONCLUSIONS: Comparing the B. vafer genome with B. pennsylvanicus and B. floridanus refines the core genes shared within the mutualist group, thereby clarifying functions required across ant host species. This third genome also allows us to track gene loss and erosion in a phylogenetic context to more fully understand processes of genome reduction.
dc.format.extent 687 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof BMC Genomics
dc.relation.isversionof 10.1186/1471-2164-11-687
dc.subject Ammonia
dc.subject Animals
dc.subject Ants
dc.subject Base Composition
dc.subject Base Sequence
dc.subject DNA, Intergenic
dc.subject Enterobacteriaceae
dc.subject Evolution, Molecular
dc.subject Frameshift Mutation
dc.subject Gene Deletion
dc.subject Genes, Bacterial
dc.subject Glutamate-Ammonia Ligase
dc.subject Metabolic Networks and Pathways
dc.subject Molecular Sequence Data
dc.subject Multigene Family
dc.subject Phylogeny
dc.subject Poly A
dc.subject Replication Origin
dc.subject Symbiosis
dc.subject Urease
dc.title Unprecedented loss of ammonia assimilation capability in a urease-encoding bacterial mutualist.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-12-2 en_US
duke.description.endpage 687 en_US
duke.description.issue en_US
duke.description.startpage 687 en_US
duke.description.volume 11 en_US
dc.relation.journal Bmc Genomics en_US
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Nicholas School of the Environment
pubs.organisational-group /Duke/Nicholas School of the Environment/Environmental Sciences and Policy
pubs.organisational-group /Duke/Trinity College of Arts & Sciences
pubs.organisational-group /Duke/Trinity College of Arts & Sciences/Biology
pubs.publication-status Published online
pubs.volume 11
dc.identifier.eissn 1471-2164

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