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Gene expression disruptions of organism versus organ in Drosophila species hybrids.

dc.contributor.author Catron, Daniel J
dc.contributor.author Noor, Mohamed AF
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:24Z
dc.date.issued 2008-08-20
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/18714377
dc.identifier.uri https://hdl.handle.net/10161/4502
dc.description.abstract Hybrid dysfunctions, such as sterility, may result in part from disruptions in the regulation of gene expression. Studies of hybrids within the Drosophila simulans clade have reported genes expressed above or below the expression observed in their parent species, and such misexpression is associated with male sterility in multigenerational backcross hybrids. However, these studies often examined whole bodies rather than testes or had limited replication using less-sensitive but global techniques. Here, we use a new RNA isolation technique to re-examine hybrid gene expression disruptions in both testes and whole bodies from single Drosophila males by real-time quantitative RT-PCR. We find two early-spermatogenesis transcripts are underexpressed in hybrid whole-bodies but not in assays of testes alone, while two late-spermatogenesis transcripts seem to be underexpressed in both whole-bodies and testes alone. Although the number of transcripts surveyed is limited, these results provide some support for a previous hypothesis that the spermatogenesis pathway in these sterile hybrids may be disrupted sometime after the expression of the early meiotic arrest genes.
dc.language eng
dc.language.iso en_US
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0003009
dc.subject Animals
dc.subject Cyclin B
dc.subject Drosophila
dc.subject Drosophila Proteins
dc.subject Gene Expression Profiling
dc.subject Gene Expression Regulation
dc.subject Male
dc.subject Nucleic Acid Hybridization
dc.subject RNA, Messenger
dc.subject Spermatids
dc.subject Spermatogenesis
dc.subject Transcription, Genetic
dc.title Gene expression disruptions of organism versus organ in Drosophila species hybrids.
dc.title.alternative
dc.type Journal article
duke.contributor.id Noor, Mohamed AF|0330154
dc.description.version Version of Record
duke.date.pubdate 2008-8-20
duke.description.issue 8
duke.description.volume 3
dc.relation.journal Plos One
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/18714377
pubs.begin-page e3009
pubs.issue 8
pubs.organisational-group Biology
pubs.organisational-group Duke
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published online
pubs.volume 3
dc.identifier.eissn 1932-6203


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