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Evolution of the sex-related locus and genomic features shared in microsporidia and fungi.

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Date
2010-05-07
Authors
Lee, Soo Chan
Corradi, Nicolas
Doan, Sylvia
Dietrich, Fred S
Keeling, Patrick J
Heitman, Joseph
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2
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Abstract
BACKGROUND: Microsporidia are obligate intracellular, eukaryotic pathogens that infect a wide range of animals from nematodes to humans, and in some cases, protists. The preponderance of evidence as to the origin of the microsporidia reveals a close relationship with the fungi, either within the kingdom or as a sister group to it. Recent phylogenetic studies and gene order analysis suggest that microsporidia share a particularly close evolutionary relationship with the zygomycetes. METHODOLOGY/PRINCIPAL FINDINGS: Here we expanded this analysis and also examined a putative sex-locus for variability between microsporidian populations. Whole genome inspection reveals a unique syntenic gene pair (RPS9-RPL21) present in the vast majority of fungi and the microsporidians but not in other eukaryotic lineages. Two other unique gene fusions (glutamyl-prolyl tRNA synthetase and ubiquitin-ribosomal subunit S30) that are present in metazoans, choanoflagellates, and filasterean opisthokonts are unfused in the fungi and microsporidians. One locus previously found to be conserved in many microsporidian genomes is similar to the sex locus of zygomycetes in gene order and architecture. Both sex-related and sex loci harbor TPT, HMG, and RNA helicase genes forming a syntenic gene cluster. We sequenced and analyzed the sex-related locus in 11 different Encephalitozoon cuniculi isolates and the sibling species E. intestinalis (3 isolates) and E. hellem (1 isolate). There was no evidence for an idiomorphic sex-related locus in this Encephalitozoon species sample. According to sequence-based phylogenetic analyses, the TPT and RNA helicase genes flanking the HMG genes are paralogous rather than orthologous between zygomycetes and microsporidians. CONCLUSION/SIGNIFICANCE: The unique genomic hallmarks between microsporidia and fungi are independent of sequence based phylogenetic comparisons and further contribute to define the borders of the fungal kingdom and support the classification of microsporidia as unusual derived fungi. And the sex/sex-related loci appear to have been subject to frequent gene conversion and translocations in microsporidia and zygomycetes.
Type
Journal article
Subject
Amino Acid Sequence
Evolution, Molecular
Fungal Proteins
Genes, Mating Type, Fungal
Genetic Linkage
Genetic Loci
Genome, Fungal
Microsporidia
Molecular Sequence Data
Phylogeny
Sequence Homology, Amino Acid
Sexual Development
Permalink
https://hdl.handle.net/10161/4539
Published Version (Please cite this version)
10.1371/journal.pone.0010539
Publication Info
Lee, Soo Chan; Corradi, Nicolas; Doan, Sylvia; Dietrich, Fred S; Keeling, Patrick J; & Heitman, Joseph (2010). Evolution of the sex-related locus and genomic features shared in microsporidia and fungi. PLoS One, 5(5). pp. e10539. 10.1371/journal.pone.0010539. Retrieved from https://hdl.handle.net/10161/4539.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Dietrich

Fred Samuel Dietrich

Associate Professor of Molecular Genetics and Microbiology
My laboratory is interested in fungal genomics.In particular we use genomic sequencing of fungal strains and species in comparative analysis. Starting with the sequencing of Saccharomyces cerevisiae strain S288C, I have been involved in the genome sequencing and annotation of Ashbya gossypii, Cryptococcus neoformans var. grubii and ~100 additional S. cerevisiae strains. We currently use Illumina paired end and mate paired sequencin
Heitman

Joseph Heitman

Chair, Department of Molecular Genetics and Microbiology
Joseph Heitman was an undergraduate at the University of Chicago (1980-1984), graduating from the BS-MS program with dual degrees in chemistry and biochemistry with general and special honors. He then matriculated as an MD-PhD student at Cornell and Rockefeller Universities and worked with Peter Model and Norton Zinder on how restriction enzymes recognize specific DNA sequences and how bacteria respond to and repair DNA breaks and nicks. Dr. Heitman moved as an E

Soo Chan Lee

Assistant Research Professor in Molecular Genetics and Microbiology
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.
Alphabetical list of authors with Scholars@Duke profiles.
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