Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates.
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Medications that can mitigate against radiation injury are limited. In this study, we investigated the ability of recombinant human growth hormone (rhGH) to mitigate against radiation injury in mice and nonhuman primates. BALB/c mice were irradiated with 7.5 Gy and treated post-irradiation with rhGH intravenously at a once daily dose of 20 microg/dose for 35 days. rhGH protected 17 out of 28 mice (60.7%) from lethal irradiation while only 3 out of 28 mice (10.7%) survived in the saline control group. A shorter course of 5 days of rhGH post-irradiation produced similar results. Compared with the saline control group, treatment with rhGH on irradiated BALB/c mice significantly accelerated overall hematopoietic recovery. Specifically, the recovery of total white cells, CD4 and CD8 T cell subsets, B cells, NK cells and especially platelets post radiation exposure were significantly accelerated in the rhGH-treated mice. Moreover, treatment with rhGH increased the frequency of hematopoietic stem/progenitor cells as measured by flow cytometry and colony forming unit assays in bone marrow harvested at day 14 after irradiation, suggesting the effects of rhGH are at the hematopoietic stem/progenitor level. rhGH mediated the hematopoietic effects primarily through their niches. Similar data with rhGH were also observed following 2 Gy sublethal irradiation of nonhuman primates. Our data demonstrate that rhGH promotes hematopoietic engraftment and immune recovery post the exposure of ionizing radiation and mitigates against the mortality from lethal irradiation even when administered after exposure.
Mice, Inbred BALB C
Mice, Inbred C57BL
Radiation Injuries, Experimental
Published Version (Please cite this version)10.1371/journal.pone.0011056
Publication InfoBourland, JD; Chao, Nelson J; Chen, BJ; Chute, JP; Cline, JM; Daher, P; ... Wang, X (2010). Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates. PLoS One, 5(6). pp. e11056. 10.1371/journal.pone.0011056. Retrieved from http://hdl.handle.net/10161/4547.
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Donald D. and Elizabeth G. Cooke Cancer Research Professor
My research interests are in two broad areas, clinical hematopoietic stem cell and cord blood transplantation and in the laboratory studies related to graft vs. host disease and immune reconstitution. On the clinical side we are currently conducting approximately 50 different clinical protocols ranging from preparatory regimens, supportive care studies and disease specific protocols. Most of these clinical studies are centered around studies of the sources of stem cells and the methods to
Adjunct Assistant Professor in the Department of Medicine
Dr. Gesty-Palmer is an Assistant Professor in the Department of Medicine and Division of Endocrinology and Metabolism. Her laboratory principally examines G protein-coupled receptor signaling mechanisms in bone metabolism with emphasis on parathyroid hormone (PTH) receptor biased agonism. These studies explore novel mechanisms of PTH receptor signaling and the contributions of β-arrestin to modulate bone formation and bone resorption. Her work has recently shown that β-arrestin,
Professor of Biostatistics and Bioinformatics
cancer pharmacogenomicsdrug induced neuropathy, neutropenia and hypertensionstatistical genetics statistical methods for high-dimensional data copulas survival analysis statistical computing
Professor in Medicine
Dr. Sempowski earned his PhD in Immunology from the University of Rochester and was specifically trained in the areas of inflammation, wound healing, and host response (innate and adaptive). Dr. Sempowski contributed substantially to the field of lung inflammation and fibrosis defining the roles of pulmonary fibroblast heterogeneity and CD40/CD40L signaling in regulating normal and pathogenic lung inflammation. During his postdoctoral training with Dr. Barton F. H
Associate Professor in Medicine
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